中国临床药理学与治疗学2017，Vol.22Issue(8)：859-865,7.
肿瘤细胞和耐药肿瘤细胞中FoxO3/Keap1/Nrf2通路的表达差异
Different expression of FoxO3/Keap1/Nrf2 pathway in tumor cells and drug resistant tumor cells
摘要
Abstract
AIM:To detect the different expression of FoxO3/Keap1/Nrf2 pathway in normal and drug resistant tumor cells so as to investigate the effect of FoxO3/Keap1/Nrf2 signal on drug resistance of tumor cell.METHODS:Cell lines of A549 (resistant to cisplatin) and HCT-8 (resistant to 5-FU) were constructed and verified by the MTT assay.The mRNA and protein expressions of FoxO3/Keapl/Nrf2 pathway members in drug-resistant tumor cells and non-drug-resistant tumor cells were evaluated by QRT-PCR and Western blot.RESULTS:The drug resistance of A549/DDP was significantly stronger than A549 cell (P ＜ 0.05),the resistance index was 5.25 times;the drug resistance of HCT-8/5-FU was significantly stronger than HCT-8 cell (P ＜ 0.05),the resistance index was 31.67 times;the mRNA expressions of FoxO3,Keapl and Akt in A549/DDP and HCT-8/5-FU cells were decreased compared with normal control cells,while mRNA expression levels of Nqo1 and Nrf2 were increased;the protein expression of FoxO3 and Keap1 in A549 DDP and HCT-8/5-FU cells were decreased compared with normal control cells,while protein expression levels of p-Akt,Nqo1 and Nrf2 were increased.CONCLUSION:FoxO3/Keap1/Nrf2 signaling pathway is related with tumor resistance,which was referential for further study of this pathway and for exploring therapeutic strategies to reduce drug resistance of cancer.关键词
FoxO3/Keap1/Nrf2信号通路/A549/HCT-8/耐药Key words
FoxO3/Keap1/Nrf2 signaling pathway/A549/HCT-8/drug resistance分类
医药卫生引用本文复制引用
叶海主,陈亚娟,赵文英,刘晓平..肿瘤细胞和耐药肿瘤细胞中FoxO3/Keap1/Nrf2通路的表达差异[J].中国临床药理学与治疗学,2017,22(8):859-865,7.基金项目
国家自然科学基金(81272485) （81272485）
