中国临床药理学杂志2017，Vol.33Issue(15)：1454-1456,1468,4.DOI:10.13699/j.cnki.1001-6821.2017.15.022
MiR-101负性调节人结肠癌细胞HCT116增殖机制的研究
Negative regulation of MiR-101 on the proliferation of colon cancer HCT116 cells and its mechanism
摘要
Abstract
Objective To study the effects and the molecular mechanism of miR-101 on the proliferation of colon cancer HCT116 cells.Methods The expression of miR-101 was regulated and treated by negative control (control group),inhibitor-miR-101 (experimental Ⅰ group),mimic-miR-101 (experimental Ⅱ group)for48 h,and the cell proli-feration was tested by [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazo-lium,MTS] assay.The Notch1 expression was also measured by Western blotting.Results The expression of miR-101 in experimental Ⅰ group,experimental Ⅱ group and control group were 0.467,1.767,0.967,respectively.The expression of miR-101 was significantly over-expressed by mimic-miR-101,or significantly inhibited by inhibitormiR-101,compared with control group,the differences were statistica-lly significant (all P ＜ 0.05).After 72 h treatment,the OD values of experimental Ⅰ group,experimental Ⅱ group and control group were 1.10,0.76,0.91.After 96 h treatment,the OD values of experimental Ⅰ group,experimental Ⅱ group and control group were 1.57,0.92,1.20.Compared with control group,the cell proliferation in experimental Ⅱ group were significantly inhibited,the differences were statistically significant (all P ＜ 0.05).The negatively regulated effect of miR-101 on the proliferation of HCT116 cell was taken by targeting Nocth1 expression.Conclusion As a cancer suppressor gene,miR-101 negatively regulated the proliferation of colon cancer HCT116 cells by targeting noth1 expression.关键词
结肠癌/微小RNA/微小RNA-101/Notch1/细胞增殖Key words
colon cancer/microRNA/MiR-101/Notch 1/cell proliferation分类
医药卫生引用本文复制引用
王园园,姜霞,肖兴春,赵春林,张强,高英超,陈琳琳,马颖玉,赵增仁..MiR-101负性调节人结肠癌细胞HCT116增殖机制的研究[J].中国临床药理学杂志,2017,33(15):1454-1456,1468,4.基金项目
科技部国际科技合作基金资助项目(2014DFA31150) （2014DFA31150）
河北省卫生和计划生育委员会基金资助项目(20150632,CY201614) （20150632,CY201614）
