中国现代医学杂志2017，Vol.27Issue(23)：18-22,5.DOI:10.3969/j.issn.1005-8982.2017.23.004
损伤软骨的细胞提取、鉴定及生物学活性研究
Isolation, identification and biological activity of chodrocytes from injured cartilage
摘要
Abstract
Objective To identify the cells isolated from injured cartilage tissue and study their growth activity, in order to provide adequate cells for autologous chondrocyte transplantation. Methods Through the knee arthroscope, a small amount of injured cartilage tissue was taken in the cartilage damage zone as experimental group, which did not include the normal cartilage or the subchondral bone. The normal cartilage was taken as control group. The cells were extracted from the experimental group, and the normal chondrocytes were extracted from the control group by trypsin and type Ⅱ collagenase. The cells of the experimental group were observed under inverted phase contrast microscope to dentify whether the cell morphology was consistent with the chondrocytes of the control group. The cells of the experimental group were identified with toluidine blue staining, and compared with the chondrocytes of the control group. Cell growth activity of the two groups was compared by MTT method. The third generation chondrocytes of the two groups were cultured on type Ⅰ/Ⅱ composite collagen membrane with patent intellectual property, and their growth activity was observed under electron microscope. Results The cells extracted from injured cartilage tissue were normal chondrocytes, and their characteristics and growth activity were not different from those of the control group ( < 0.05). Conclusions In the process of cartilage injury, the cartilage cells do not degenerate. Appropriate amount of chondrocytes can be obtained from a small amount of injured cartilage tissue, it is proved that the growth activity is in accordance with the requirements of autologous chondrocyte transplantation by MTT method and electron microscopy. As a result, we do not need the normal cartilage tissue as a cell source.关键词
损伤软骨/软骨细胞/自体软骨细胞移植Key words
injured cartilage/chondrocyte/autologous chondrocyte transplantation分类
医药卫生引用本文复制引用
姚旺林,刘理金,徐训安,丛波,张加廷,张仲文..损伤软骨的细胞提取、鉴定及生物学活性研究[J].中国现代医学杂志,2017,27(23):18-22,5.基金项目
首都临床特色应用研究(No:Z161100000516013) （No:Z161100000516013）
