中国药房2017，Vol.28Issue(28)：3900-3903,4.DOI:10.6039/j.issn.1001-0408.2017.28.04
丹酚酸B抗心肌纤维化的机制研究
Study on the Mechanism of Anti-myocardial Fibrosis of Salvianolic Acid B
摘要
Abstract
OBJECTIVE:To study the effects of salvianolic acid B(Sal B)on angiotensin Ⅱ(Ang Ⅱ)-induced cardiac fibro-blast proliferation,secretion of type Ⅲ collagen,protein expressions of matrix metalloproteinase 9 (MMP-9),Smad2/3,Smad7, and explore its mechanism of anti-myocardial fibrosis. METHODS:Cells were divided into blank control group(culture medium) Ang Ⅱ model group,Sal B low-dose,medium-dose,high-dose groups (12.5,25,50 μmol/L). After cultured 1 h by blank or drug-containing culture,except for blank control group,cells in other groups were added 1 μmol/L Ang Ⅱ to induce proliferation. for 24 h. MTT method and hematoxylin-eosin staining method were adopted investigate the effect of Sal B on proliferation. Western blot method was adopted to detect the effects of Sal B on protein expressions of type Ⅲ collagen,MMP-9,Smad2/3,Smad7. RE-SULTS:Compared with blank control group,cells in Ang Ⅱ model group were significantly proliferated,protein expressions of type Ⅲ collagen,MMP-9,Smad2/3 were obviously enhanced,protein expression of Smad7 was obviously weakened,with statisti-cal significances(P<0.05). Compared with Ang Ⅱ model group,the cell proliferation in Sal B groups were inhibited,protein ex-pressions of typeⅢcollagen,MMP-9,Smad2/3 were weakened,while protein expression of Smad7 was enhanced. Except the pro-tein expression of type Ⅲ collagen in Sal B low-dose and medium-dose groups,the protein expression of Smad2/3 in Sal B high-dose group did not change significantly,other indexes had statistical significances(P<0.05). CONCLUSIONS:The anti-myo-cardial fibrosis effect of Sal B may be associated with inhibiting the proliferation of cardiac fibroblasts,down-regulating protein ex-pressions of typeⅢcollagen,MMP-9,Smad2/3 and up-regulating protein expression of Smad7.关键词
丹酚酸B/心肌成纤维细胞/Ⅲ型胶原/基质金属蛋白酶9/转化生长因子β1信号通路Key words
Salvianolic acid B/Cardiac fibroblasts/Type Ⅲ collagen/Matrix metalloproteinase 9/Transforming growth factorβ1 signaling pathway分类
医药卫生引用本文复制引用
罗红,王春花,赵玲璐,杨宇,陈世平,徐旖旎,杨红宇,沈祥春..丹酚酸B抗心肌纤维化的机制研究[J].中国药房,2017,28(28):3900-3903,4.基金项目
国家自然科学基金资助项目(No.81560588) （No.81560588）
贵州省科技合作计划项目(No.黔科合LH字〔2016〕7356号) （No.黔科合LH字〔2016〕7356号）
贵州省科学技术基金项目(No.黔科合JZ字〔2015〕2002号) （No.黔科合JZ字〔2015〕2002号）
贵州省高层次创新型人才培养项目(No.黔科合人才〔2015〕4029号) （No.黔科合人才〔2015〕4029号）
贵州医科大学2015年高等学校大学生创新创业训练计划项目(No.201510660007) （No.201510660007）
