中国药房2017，Vol.28Issue(28)：3935-3937,3.DOI:10.6039/j.issn.1001-0408.2017.28.13
蕲蛇煮散剂的最佳粉碎粒度优选及其对人类风湿性关节炎成纤维样滑膜细胞凋亡的影响
Optimization of the Best Crushing Particle Size of Agkistrodon Decocting Powder and Its Effect on Apopto-sis of Human Rheumatoid Arthritis Fibroblast-like Synoviocytes
摘要
Abstract
OBJECTIVE:To optimize the best crushing particle size of Agkistrodon decocting powder,and study its effect on apoptosis of human rheumatoid arthritis fibroblast-like synoviocytes. METHODS:Pre-column derivatization RP-HPLC was adopted. Using the total decoction amounts of 4 main amino acids (aspartic acid,glutamic acid,L-hydroxyproline,glycine) that decocted once as index,the Agkistrodon decoction pieces and those through No.1-8 seive were screened,and the best crushing particle size of Agkistrodon decocting powder was optimized. The human rheumatoid arthritis fibroblast-like synoviocytes were divided into nega-tive control group,positive control group (1 μmol/L methotrexate) and the best crushing particle size of Agkistrodon decocting powder group(2.0 mg/mL),flow cytometry was used to determine the cell apoptosis after cultured for 48 h. RESULTS:The best crushing particle size was through No.6 seive,when the total amount of 4 main amino acids was(61.27±0.02)mg/g(n=3). Com-pared with negative control group,the apoptosis rate in the best crushing particle size of Agkistrodon decocting powder group was significantly increased(P<0.05),which was slightly higher than positive control group. CONCLUSIONS:The best crushing parti-cle size is through No.6 seive;and Agkistrodon decocting powder shows effect on reducing the apoptosis of human rheumatoid ar-thritis fibroblast-like synoviocytes.关键词
蕲蛇/煮散剂/粉碎粒度/人类风湿性关节炎成纤维样滑膜细胞/凋亡Key words
Agkistrodon/Decocting powder/Crushing particle size/Human rheumatoid arthritis fibroblast-like synoviocytes/Apoptosis分类
医药卫生引用本文复制引用
刘芳,董改英,瞿晶田,柴士伟..蕲蛇煮散剂的最佳粉碎粒度优选及其对人类风湿性关节炎成纤维样滑膜细胞凋亡的影响[J].中国药房,2017,28(28):3935-3937,3.基金项目
天津市中医中西医结合科研课题(No.13060) （No.13060）
