中国药房2017，Vol.28Issue(28)：3938-3941,4.DOI:10.6039/j.issn.1001-0408.2017.28.14
光裸方格星虫纤溶酶SNFE的抗凝作用及机制研究
Study on the Anti-coagulation Effect and Mechanism of Fibrinolytic Enzyme SNFE in Sipuculus Nudus
摘要
Abstract
OBJECTIVE:To study the anti-coagulation effect and mechanism of fibrinolytic enzyme SNFE in sipuculus nudus, and provide reference for further development of SNFE. METHODS:40 mice were randomly divided into blank control group(nor-mal saline),Xueshuantong group(positive control,15 mg/kg)and SNFE low-dose,high-dose group(15,30 mg/kg),10 in each group. After intravenous injection in tail,tail bleeding time (BT) and clotting time (CT) were respectively determined to investi-gate the anti-coagulation effect of SNFE. After taking blood in abdominal aorta of rats,test was divided into blank control group, positive control group and SNFE low-mass concentration,medium-mass concentration,high-mass concentration groups (0.25, 0.50,1.00 mg/mL). Prothrombin time(PT),re-calcium time(PRT)(using orokinase as positive drug,100000 U/mL),and max-mum platelet aggregation rate (PAG) in 5 min under adenosine diphosphate (ADP) inducer (using asprin as positive drug,0.50 mg/mL) were respectively determined,and anti-coagulation effect mechanism of SNFE was analyzed. RESULTS:Compared with blank control group,BT,CT of mice in each group were prolonged,with statistical significance in Xueshuantong group and SNFE high-dose group (P<0.05 or P<0.01). Plasma PT of rats in positive control group,SNFE medium-dose,high-dose groups and PRT in each administration group were significantly prolonged(P<0.05 or P<0.01);and PAG in administration group was signifi-cantly reduced(P<0.01). CONCLUSIONS:The fibrinolytic enzyme SNFE in sipuculus nudus can play its anti-coagulant effect by inhibiting the activity of coagulation factors in internal and external sources and ADP-induced platelet aggregation.关键词
光裸方格星虫/纤溶酶/抗凝作用/血小板聚集Key words
Sipuculus nudus/Fibrinolytic enzyme/Anti-coagulation effect/Platelet aggregation分类
医药卫生引用本文复制引用
李映新,黄晓亮,黄媛恒,班建东,庞丽君,黎钦蓉,雷丹青..光裸方格星虫纤溶酶SNFE的抗凝作用及机制研究[J].中国药房,2017,28(28):3938-3941,4.基金项目
广西医科大学青年科学基金项目(No.GXMUYSF-2014025) （No.GXMUYSF-2014025）
广西高校-广西再生医学重点实验室开放课题(No.桂再重开15-05) （No.桂再重开15-05）
