中国药房2017，Vol.28Issue(28)：3964-3968,5.DOI:10.6039/j.issn.1001-0408.2017.28.21
响应面法优化栀子中总多酚、总黄酮的提取工艺
Optimization of Extraction Technology of Total Polyphenols and Total Flavonoids in Gardenia jasminoides by Response Surface Method
摘要
Abstract
OBJECTIVE:To optimize the extraction technology of total polyphenol and total flavonoid in Gardenia jasminoi-des. METHODS:Plackett-burman(PB)design was used to select the ethanol volume fraction,liquid-solid ratio,particle size,ex-traction time and extraction temperature to determine the key factor affecting the extraction of total polyphenol and total flavonoids in G. jasminoides. Then central composite design (CCD) was combined with response surface method to optimize the extraction technology,and verification test was conducted. RESULTS:The optimal extraction conditions of total polyphenol were 40% etha-nol,particle size of 0.20 mm,extraction temperature of 60 ℃,liquid-solid ratio of 20,and extraction time of 20 min;the optimal extraction conditions of total flavonoids were 40% ethanol,particle size of 0.20 mm,extraction temperature of 30 ℃,liquid-solid ratio of 20,and extraction time of 20 min. In verification test,the contents of total polyphenol and total flavonoids in G. jasminoi-des were 1.70%(RSD=1.43%,n=3),3.23%(RSD=3.72%,n=3),with relative error of 1.80%,8.75% with predicted val-ues,respectively. CONCLUSIONS:The response surface method based on PB and CCD is simple,reasonable and feasible to opti-mize the extraction technology of total polyphenol and total flavonoids in G. jasminoides. The method can provide reference for its industrial extraction.关键词
栀子/多酚/黄酮/Plackett-burman设计/星点设计/响应面法/提取工艺/优化Key words
Gardenia jasminoides/Polyphenol/Flavonoid/Plackett-burman design/Central composite design/Response sur-face method/Extraction technology/Optimization分类
医药卫生引用本文复制引用
黄潇,刘婧,彭水梅,刘德鸿,付小梅,裴建国,吴志瑰..响应面法优化栀子中总多酚、总黄酮的提取工艺[J].中国药房,2017,28(28):3964-3968,5.基金项目
国家自然科学基金资助项目(No.81360620) （No.81360620）
