中成药2017，Vol.39Issue(9)：1771-1776,6.DOI:10.3969/j.issn.1001-1528.2017.09.003
牛耳枫提取物的抗炎作用
Anti-inflammatory effects of Daphniphyllum calycinum extract
宋青 1朱粉霞 2李冬玉 2张浩 3陈家金2
作者信息
- 1. 西安交通大学第一附属医院妇产科,陕西西安710061
- 2. 江苏省中医药研究院,国家中医药管理局中药释药系统重点研究室,江苏南京210028
- 3. 中国药科大学分析化学教研室,江苏南京210009
- 折叠
摘要
Abstract
AIM To study the in vitro and in vivo anti-inflammatory effects of extract from Daphniphyllum calycinum Benth and its mechanism of action.METHODS LPS-induced mouse RAW264.7 macrophage and mouse endotoxemia model were used to observe the influences of D.calycinum extract on the levels of NO,TNF-α,IL-1β and IL-10.The toxicity of D.calycinum extract on RAW264.7 macrophage was determined by MTT assay.Fifty female BALB/c mice were administered with D.calycinum extract by gavage.Fourteen days later,the mice were intraperitoneally injected with LPS,the levels of NO,TNF-α,IL-1 β and IL-10 in serum were determined by Griess and ELISA kit.The protein expressions of NO and TNF-α in mouse liver were studied by immunohistochemistry method.RESULTS D.calycinum extract could significantly inhibit the release of NO,TNF-α,IL-1 β and IL-10.Immunohistochemistry results showed that the extract could inhibit the protein expressions of iNOS and TNF-α.CONCLUSION D.calycinum extract has a significant anti-inflammatory effect,and its mechanism may be related to decreasing the release of inflammatory factors.关键词
牛耳枫/抗炎/脂多糖/RAW264.7巨噬细胞/内毒素血症Key words
Daphniphyllum calycinum Benth/anti-inflammatory/lipopolysaccharide/RAW264.7 macrophage/endotoxemia分类
医药卫生引用本文复制引用
宋青,朱粉霞,李冬玉,张浩,陈家金..牛耳枫提取物的抗炎作用[J].中成药,2017,39(9):1771-1776,6.
