中成药2017，Vol.39Issue(9)：1782-1788,7.DOI:10.3969/j.issn.1001-1528.2017.09.005
补肾活血方对卵巢早衰小鼠颗粒细胞TGF-β1、TGF-βRⅡ、Smad2/3表达的影响
Effects of Bushen Huoxue Recipe on the expressions of TGF-β1, TGF-βRⅡ and Smad2/3 in granulosa cells in mice with premature ovarian failure
摘要
Abstract
AIM To explore the effects of Bushen Huoxue Recipe (Fluoritum,Psoraleae Fructus,Cuscutae Semen,etc.) on the protein expressions of transforming growth factor (TGF)-β1,TGF-βRⅡ and Smad2/3 in follicle wall granulosa cells in mice with autoimmune premature ovarian failure (POF).METHODS Balb/c female mice were subcutaneously injected with mouse zona pellucida 3 at multiple points to establish autoimmune POF model.POF mice were divided into model group,positive group (progynova),low-,middle-and high-dose of Bushen Huoxue Recipe groups.After intervention for 30 days,ovarian tissue was stained by hematoxylin-eosin (HE),and the protein expressions of TGF-β1,TGF-βRⅡ and Smad2/3 in follicle wall granulosa cells and ovarian tissue were detected by immunohistochemistry and Western blot,respectively.RESULTS Compared with the model group,the number of mature follicles in the Bushen Huoxue Recipe groups and the positive group was obviously increased,together with the decreased number of atretic follicles.The protein expressions of TGF-β1,TGF-βRⅡ and Smad2/3 in follicle wall granulosa cells and ovarian tissue in the Bushen Huoxue Recipe groups and the positive group were much higher than those in the model group (P ＜ 0.05).CONCLUSION Bushen Huoxue Recipe can improve ovarian function by up-regulating the protein expressions of TGF-β1,TGF-βRⅡ and Smad2/3 in granulosa cells.关键词
补肾活血方/卵巢早衰/卵泡壁颗粒细胞/TGF-β1/TGF-βRⅡ/Smad2/3Key words
Bushen Huoxue Recipe/premature ovarian failure/follicle wall granulosa cells/TGF-β1/TGF-βRⅡ/Smad2/3分类
医药卫生引用本文复制引用
刘慧萍,曾柳庭,胡立娟,王灿,杨凯麟,张国民..补肾活血方对卵巢早衰小鼠颗粒细胞TGF-β1、TGF-βRⅡ、Smad2/3表达的影响[J].中成药,2017,39(9):1782-1788,7.基金项目
国家自然基金项目(81303123) （81303123）
湖南省教育厅创新平台项目(15K090) （15K090）
湖南省重点实验室开放基金(2014XNFZ06) （2014XNFZ06）
湖南省大学生创新项目(2016-281) （2016-281）
国家级大学生创新项目(201510541002) （201510541002）
