中成药2017，Vol.39Issue(9)：1813-1819,7.DOI:10.3969/j.issn.1001-1528.2017.09.010
三物黄芩汤组分(群)配伍在大鼠肝微粒体孵育模型中的相互作用
Interactions of component (group) compatibility of Sanwu Huangqin Decoction in incubation model of rat liver microsomes
摘要
Abstract
AIM To study the interactions of component (group) compatibility of Sanwu Huangqin Decoction in incubation model of rat liver microsomes.METHODS With maackiain and ephedrine as internal standards,HPLC was adopted in the simultaneous content determination of total tlavonoids (baicalin,wogonoside,baicalein,wogonin and oroxylin A) from Scutellariae Radix and total alkaloids (oxymatrine,oxysophocarpine and matrine)from Sophorae flavescentis Radix in whole prescription component (group) compatibility and single medicinal material part (group) at seven time points (0,15,30,45,60,90 and 120 min),followed by the calculation of their in vitro metabolic rates.RESULTS In whole prescription component (group) compatibility,the contents of baicalin and wogonoside were first increased (0-0.5 h) and then decreased (0.5-2.0 h),those of baicalein,wogonin and oroxylin A showed increasing trends (more obvious within 0.5 h).The stable and gende metabolisms of various alkaloids reached balance within 20 min.CONCLUSION The bioavailability improvement and efficacy enhancement of total flavonoids from Scutellariae Radix may attribute to the compatibility with total alkaloids from Sophorae flavescentis Radix and total polysaccharides from Rehmanniae Radix in Sanwu Huangqin Decoction关键词
三物黄芩汤/组分(群)配伍/黄芩总黄酮/苦参总生物碱/地黄总多糖/代谢/大鼠肝微粒体/HPLCKey words
Sanwu Huangqin Decoction/component (group) compatibility/total flavonoids from Scutellariae Radix/total alkaloids from Sophorae flavescentis Radix/total polysaccharides from Rehmanniae Radix/metabolisms/rat liver microsomes/HPLC分类
医药卫生引用本文复制引用
周杰,商雪莹,杜慧琴,卢丽萍,张蕾..三物黄芩汤组分(群)配伍在大鼠肝微粒体孵育模型中的相互作用[J].中成药,2017,39(9):1813-1819,7.基金项目
广东省科技计划项目(2016A020226031) （2016A020226031）
广州市科技计划项目(201607010334) （201607010334）
青年英才培育项目(A1-AFD015131Z08) （A1-AFD015131Z08）
