中成药2017，Vol.39Issue(9)：1819-1824,6.DOI:10.3969/j.issn.1001-1528.2017.09.011
薯蓣皂苷元纳米混悬液的制备
Preparation of diosgenin nanosuspensions
摘要
Abstract
AIM To prepare diosgenin nanosuspensions.METHODS The nanosuspensions prepared by media milling method were solidificated by freeze drying method.With particle size and polydispersity index (PDI) as evaluation indices,stabilizer kind,ratio of diosgenin to stabilizer,ratio of preliminary nanosuspension volume to grinding bead amount,milling time,lyoprotectant kind and its amount as influencing factors,single factor test was applied to screening preparation and solidification processes.The morphology of nanosuspensions was observed,then the particle sizes and polydispersity indices of nanosuspensions and freeze-dried powder were determined.RESULTS The optimal conditions were determined to be 6:1 for ratio of diosgenin to Pluronic F127 (stabilizer Ⅰ),50:1 for ratio of diogenin to sodium dodecyl sulfate (SDS,stabilizer Ⅱ),1:4 for ratio of preliminary nanosuspension volume to grinding bead amount,120 min for milling time,and 8％ PEG-6000 and 2％ mannitol as lyoprotectants.The average particle size and polydispersity index of rod-like or flaky nanosuspensions were (348.1 ±14.2) nm and 0.244 ± 0.059,respectively,which were lower than those of freeze-dried powder.At room temperature,the particle sizes of nanosuspensions and freeze-dried powder remained stable within 35 d and 3 months,respectively.CONCLUSION The physical stability of diosgenin freeze-dried powder is superior to that of its nanosuspensions,which can be used after being reconstituted.关键词
薯蓣皂苷元/纳米混悬液/介质研磨/冷冻干燥/单因素试验Key words
diosgenin/nanosuspensions/media milling/freeze drying/single factor test分类
医药卫生引用本文复制引用
常金花,薛禾菲,刘沛,刘翠哲..薯蓣皂苷元纳米混悬液的制备[J].中成药,2017,39(9):1819-1824,6.基金项目
河北省自然科学基金资助项目(H2014406036) （H2014406036）
河北省高等学校科学技术青年基金项目(QN2015127) （QN2015127）
河北省高校重点学科建设项目(冀教高[2013]4) （冀教高[2013]4）
