中成药2017，Vol.39Issue(9)：1841-1844,4.DOI:10.3969/j.issn.1001-1528.2017.09.016
HPLC-ELSD法同时测定天泰1号片中8种成分
Simultaneous determination of eight constituents in Tiantai No.1 Tablets by HPLC-ELSD
摘要
Abstract
AIM To establish an HPLC-ELSD method for the simultaneous content determination of gastrodin,parishin A,parishin B,ginsenosides Rg1,Re,Rf,Rb1 and Rd in Tiantai No.1 Tablets (Ginseng Radix et Rhizoma,Gastrodiae Rhizoma,Cistanches Herba,etc.).METHODS The analysis of methanol extract of this drug was performed on a 45 ℃C thermostatic Alltima C1scolumn (4.6 mm × 250 mm,5 μm),with the mobile phase comprising of acetonitrie-0.1％ phosphoric acid flowing at 1.0 mL/min in a gradient elution manner.RESULTS Eight constituents showed good linear relationships within their own ranges (r ＞ 0.999 0),whose average recoveries were 96.94％-97.93％ with the RSDs of 1.06％-2.48％.CONCLUSION This simple,accurate and reliable method can be used for the quality control of Tiantai No.1 Tablets.关键词
天泰1号片/化学成分/HPLC-ELSDKey words
Tiantai No.1 Tablets/chemical constituents/HPLC-ELSD分类
医药卫生引用本文复制引用
梁少瑜,曾永长,谭晓梅,吴正治..HPLC-ELSD法同时测定天泰1号片中8种成分[J].中成药,2017,39(9):1841-1844,4.基金项目
中国博士后科学基金项目(2016M592604) （2016M592604）
广东省科技厅公益研究与能力建设专项资金新药创制专题项目(2015B020211001) （2015B020211001）
深圳市科技计划项目(JCYJ20160428105555220) （JCYJ20160428105555220）
广东省中医药科研项目(20171235) （20171235）
