中成药2017，Vol.39Issue(9)：1873-1876,4.DOI:10.3969/j.issn.1001-1528.2017.09.023
UPLC法同时测定香椿芽中8种黄酮醇苷
Simultaneous determination of eight flavonol glycosides in Toona sinensis shoots by UPLC
摘要
Abstract
AIM To establish a UPLC method for the simultaneous content determination of glycosides,rutinoside,myricitrin,hyperoside,isoquercitrin,guaijaverin,astragalin,quercitrin and afzelin in the shoots of Toona sinensis Roemer.METHODS The analysis of 60％ methanol extract of T.sinensis shoots was performed on a 35 ℃ thermostatic Waters XBridge Shield RP18 column (2.1 mm × 150 mm,1.7 μm),with the mobile phase comprising of acetonitrile-water flowing at 0.35 mL/min in a gradient elution manner,and the detection wavelength was set at 350 nm.RESULTS Eight flavonol glycosides showed good linear relationships within their own ranges (r≥ 0.9992),whose average recoveries were 98.3％-103.5％ with the RSDs of 0.46％-3.36％.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of T.sinensis shoots.关键词
香椿/芽/黄酮醇苷/UPLCKey words
Toona sinensis Roemer/shoots/flavonol glycosides/UPLC分类
医药卫生引用本文复制引用
葛重宇,林玲,顾芹英,李楠,杨欢,贾晓斌..UPLC法同时测定香椿芽中8种黄酮醇苷[J].中成药,2017,39(9):1873-1876,4.基金项目
国家自然科学基金(81303313) （81303313）
江苏省自然科学基金(BK20150463) （BK20150463）
江苏省“六大人才高峰”项目(YY-010) （YY-010）
江苏省药学会-奥赛康医院药学基金(A201619) （A201619）
江苏大学学生科研立项(14A145) （14A145）
