中药材2017，Vol.40Issue(4)：762-768,7.DOI:10.13863/j.issn1001-4454.2017.04.002
通过改变土壤pH及菌群结构缓解杭白菊连作障碍
Alleviating Replant Disease of Chrysanthemum morifolium by Changing the Soil pH and Bacterial Structure
摘要
Abstract
Objective:To investigate the way to alleviate the replant disease of Chrysanthemum morifolium,and to explore its microecological mechanism.Methods:The experiment was divided into six groups,continuous cropping group,rotation group,silkworm fermented fertilizer group,silkworm excrement group,commercial fertilizer group and lime group.The survival rate,yield,plant height and the number of branches were analyzed,and the rhizosphere soil bacterial community diversity and structure of Chrysanthemum morifolium were analyzed by Miseq sequencing technique.Results:The survival rate,yield,plant height and the number of branches were the lowest,and the soil acidification was severe,the pH was 4.79,and the rhizophere soil enzyme activity and bacterial diversity was the lowest.The survival rate,yield,plant height and branches number of the Chrysanthemum morifolium were significantly improved by the application of silkworm fermented fertilizer,silkworm excrement,commercial fertilizer and lime.Compared with the physical and chemical properties of soil,there was no significant difference in organic matter content between the application of bacterial fertilizer and lime,while the soil pH,enzyme activity and bacterial diversity were increased significantly.Compared with the top 20 dominant species,there was no significant difference in the dominant species of three fertilization groups,mainly in the content difference.The application of fertilizers promoted the growth of some dominant bacterial groups in the soil,such as Gp1,Rhizomicrobium,Pseudolabrys,Gp2,Burkholderia,Bradyrhizob ium,Devosia,Bacillus,Sphingomonas,Chitinophag a,among them Devosia,Bradyrhizobium,Bacillus,Burkholderia,Sphingomonas and Chitinophaga are probiotics.The bacterial composition in the lime group was different from that in the bacterial fertilizer group.Some of the probiotics such as Arthrobacter,Paenibacillus,Nitrosospira,Ohtaekwangia,Tepidamorphus and Dongia were only present in the lime group.Conclusion:Whether the fertilization or the change of soil pH can improve the yield and increase the survival rate of Chrysanthemum morifolium,the microecological mechanism is mainly to improve the soil pH,enzyme activity and bacterial diversity,increase the number and quantity of beneficial bacteria,and then to achieve the effect of alleviating the obstruction of Chrysanthemum morifolium.关键词
杭白菊/Miseq测序/根际微生物/连作/蚕沙发酵有机肥Key words
Chrysanthemum morifolium Ramat./Miseq sequencing/Rhizo-microbe/Continuous cropping/Silkworm fermented fertilizer分类
医药卫生引用本文复制引用
倪方方,彭三妹,徐建中,王博林,徐红梅,宋腾蛟,袁小凤..通过改变土壤pH及菌群结构缓解杭白菊连作障碍[J].中药材,2017,40(4):762-768,7.基金项目
浙江省自然科学基金(LY16H280006) （LY16H280006）
