中药材2017，Vol.40Issue(4)：789-796,8.DOI:10.13863/j.issn1001-4454.2017.04.008
天麻苯丙烷代谢途径的转录组学分析
Transcriptional Analysis of Phenylpropanoid Metabolic Pathway in Gastrodia elata
摘要
Abstract
Objective:To study the relationship between the content of secondary metabolites and the activities of related enzymes in Gastrodia elata based on transcriptome analysis.Methods:Hiseq Illumina 4000 was used to detect the sequencing analysis of the fresh and post harvest five days Gastrodia elata,and with gene database compared to annotate and find differentially expressed genes.Results:18 772 600,19 353 329 Clean Reads were obtained respectively,and 63 455 Unigene was got after Trinity assembly.Through the NR,Swiss-Prot,GO,COG,KOG and KEGG database comparison 18 913 annotated Unigene finally.Total 2 829 differentially expressed genes were found after five days,including expression increased genes 953,and expression decreased genes 1 876.634 differentially expressed genes were annotated by COG into 25 functional categories,and 612 differentially expressed genes were annotated by KEGG into 50 metabolic pathways.Eleven key genes of Gastrodia elata differential expression data were annotated by the KEGG database into phenylpropanoid metabolic pathway.Compared with fresh G astrodia elata,the expression of CM,PDT,PAL,4CL,CHS,F5 H and F3'M decreased,and the expression of C4H and CCR increased after five days preservation,and CAD expression both increased and decreased.Conclusion:This study has obtained a complete metabolic pathway map of the product of Gastrodia elata,and annotated a large number of related gene sequences.The research has laid a certain reference basis for the follow-up study of the gene function and the molecular mechanism of quality formation of Gastrodia elata,the quality control of the cultivation process and the preservation of the post harvest.关键词
天麻/转录组/差异表达基因/苯丙烷类代谢Key words
Gastrodia elata Bl./Transcriptome/Differentially expressed genes/Phenylpropanoid metabolic pathway分类
医药卫生引用本文复制引用
文欢,张大燕,彭成,王伟,谢晓芳,饶朝龙,高继海..天麻苯丙烷代谢途径的转录组学分析[J].中药材,2017,40(4):789-796,8.基金项目
广元川产道地药材天麻杜仲规范化种植与精深加工技术集成示范(313-877) （313-877）
中药资源四川省青年科技创新研究团队(319-630) （319-630）
