中药材2017，Vol.40Issue(4)：869-872,4.DOI:10.13863/j.issn1001-4454.2017.04.025
UPLC-MS/MS法测定桂林产苦苣苔科植物中苯乙醇苷类成分含量
Determination of Seven Kinds of Phenylethanol Glycosides in Gesneriaceae Species from Guilin Habitats by UPLC-MS/MS
摘要
Abstract
Objective:To establish an UPLC-MS/MS method for the simultaneous determination of seven phenylethanol glycosides in Gesneriaceae species,and to analyze the difference of phenylethanol glycosides ingredients in the samples from Guilin habitats.Methods:The determination was performed on the AQUITY BEH C18 (100 mm × 2.1 mm,1.8 μμm);the mobile phase was 0.1％ formic acid (A)-0.1％ formic acid acetonitrile (B) in gradient elution;the flow rate was 0.6 mL/min;the column temperature was 40 ℃.MS instrument was equipped with ESI-ion source.Gaining the extracted ion chromatograms,then the peak area was used for quantitative.Results:Seven phenylethanol glycosides showed good linearity(r≥0.9990).The RSD of the precision,repeatability,and stability tests were less than 3.5％.The average recovery rates were in the range of 97.1％ ～ 103.2％,the RSD were less than 3.6％.There are differences in the composition and content of seven phenylethanol glycosides from different Gesneriaceae species.Conclusion:The method for the simultaneous determination of the seven phenylethanol glycosides is simple,accurate,and reproducible,which has great theoretical significance for looking for high levels of phenylethanol glycosides of plant resources.关键词
苦苣苔科植物/苯乙醇苷类/UPLC-MS/MSKey words
Gesneriaceae species/Phenylethanol glycosides/UPLC-MS/MS分类
医药卫生引用本文复制引用
张瑜,杨健,詹志来,胡峻,刘勇,袁媛..UPLC-MS/MS法测定桂林产苦苣苔科植物中苯乙醇苷类成分含量[J].中药材,2017,40(4):869-872,4.基金项目
国家自然科学基金(81373959) （81373959）
中医药行业科研专项(201407003) （201407003）
2016年北京中医药大学自主选题(在读研究生项目)(2016-JYB-XS125) （在读研究生项目）
