安徽农业科学2017，Vol.45Issue(27)：1-3,3.
基于大鼠脑缺血再灌注损伤模型建立芍药内酯苷的PK-PD模型
Pharmacokinetic-Pharmacodynamic Link Model of Albiflorin Based on Cerebral Ischemia-Reperfusion Injury Model Rats
摘要
Abstract
[Objective] The aim was to establish the pharmacokinetic-pharmacodynamic(PK-PD) model of albiflorin in Xinshao formula. [ Method] Firstly, in order to get the drug-time curve of albiflorin in Xinshao formula in plasma collected from different time points of a disease model animal, whose concentration were determined by the method of UPLC-MS.At the same time, the content of SOD and LDH in the plas-ma samples of different time points were determined by the kit for time-effect curve.Then the pharmacokinetic software was used to fit the pharmacokinetic parameters of albiflorin with the compartmental model and to get PK parameters.After that, the pharmacokinetic parameters were fixed, and the time effect relationship were fitted to get the relevant PD parameters.According to the PD parameters, the PK-PD model of albiflorin had been established .[Result] When SOD was used as the index of efficacy , the PK-PD model of albiflorin was obtained as E=21.04+(7.16 ×Ce)/(Ce+372.4);When LDH was used as the index of efficacy , the PK-PD model of albiflorin was obtained as E=216.83-(37.31 × Ce)/(Ce+0.04).[Conclusion] The PK-PD model of albiflorin in the formula showed that the concentration of SOD and LDH was correlated with the concentration of albiflorin .Albiflorin could protect cerebral ischemia reperfusion injury by increasing SOD and decreasing LDH .关键词
大鼠脑缺血再灌注损伤模型/辛芍组方/芍药内酯苷/PK-PD模型Key words
Cerebral Ischemia-Reperfusion Injury Model Rats/Xinshao formula/Albiflorin/PK-PD model分类
医药卫生引用本文复制引用
巩仔鹏,李梅,胡建春,吴林霖,王爱民,李勇军,李月婷..基于大鼠脑缺血再灌注损伤模型建立芍药内酯苷的PK-PD模型[J].安徽农业科学,2017,45(27):1-3,3.基金项目
国家自然科学基金项目(81260636) （81260636）
贵州省优秀青年科技人才培养项目(黔科合人字〔2015〕11号) （黔科合人字〔2015〕11号）
贵州省民族药药效物质基础研究科技创新人才团队\贵州省高层次创新型人才培养(百层次黔科合平台人才〔2016〕5613\5677) （百层次黔科合平台人才〔2016〕5613\5677）
贵州省中医药管理局项目(QZZY-2015-080). （QZZY-2015-080）
