安徽农业科学2017，Vol.45Issue(27)：10-11,16,3.
常压室温等离子体(ARTP)对脱落酸产生菌的诱变效应
Mutagenic Effects of Atmospheric and Room Temperature Plasma (ARTP) on Abscisic Acid (ABA) Producing Strains
毛玉华 1张金儿 1周义凤1
作者信息
- 1. 江西新瑞丰生化股份有限公司,江西新干331300
- 折叠
摘要
Abstract
[Objective] The aim was to study mutagenic effects of atmospheric and room temperature plasma (ARTP) on abscisic acid (ABA) producing strains to obtain mutants with high ABA production.[ Method] ARTP was employed to generatethe mutants under the conditions of 100 W power supply,the plasma temperature of 25℃,gas flow of 10 SLM,and irradiation distance of 2 mm.The spore of Botrytis cinerea was trea-ted by different mutation time.The statistical analysis of the mutagenic effects was carried out in terms of lethality ,positive mutation rate and am-plitude of variation.[Result] The positive mutation rate was above 20%and amplitude of variation was-30.53%-22.06%under the condition of 100 s treatment time with ARTP.The high-yield mutant 6-2 had an increase of 22.06% comparing with the original strain,and the yield did not change significantly after five consecutive passage tests.[ Conclusion] The ARTP mutation method has a high positive mutation rate and better stability on ABA producing strains.The stability of high-yield mutant is good.关键词
脱落酸/常温室压等离子体/诱变效应Key words
Abscisic acid/ARTP/Mutagenic effects分类
农业科技引用本文复制引用
毛玉华,张金儿,周义凤..常压室温等离子体(ARTP)对脱落酸产生菌的诱变效应[J].安徽农业科学,2017,45(27):10-11,16,3.
