安徽农业科学2017，Vol.45Issue(28)：1-2,35,3.
枯草芽孢杆菌发酵产N-乙酰神经氨酸的分离提纯
Separation and Purification of N-acetylneuraminic Acid Produced by Bacillus subtilis
摘要
Abstract
[Objective] The aim was to separate and purify of N-acetylneuraminic acid from Bacillus subtilis which has a Generally Recognized as Safe (GRAS) status to get the safe and good production.[Method] After the fermentation of Bacillus subtilis in right medium,N-acetylneuraminic acid,was obtained by eradicating cell wall in boiling water,separating impurity,decolorization process,ethanol precipitation,acetic acid crystalli-zation,recrystallization and other steps.[Result] Through the high performance liquid chromatography (HPLC) detection,the purity of N-acetyl-neuraminic acid reached above 96.0%.[Conclusion] The results provide reference for the application of N-acetylneuraminic acid in health prod-ucts and other fields.关键词
枯草芽孢杆菌/N-乙酰神经氨酸/纯化Key words
Bacillus subtilis/N-acetylneuraminic acid/Purification分类
农业科技引用本文复制引用
高兰兰,袁丽霞,吴金勇,陈祥松,孙立洁,朱微微,姚建铭..枯草芽孢杆菌发酵产N-乙酰神经氨酸的分离提纯[J].安徽农业科学,2017,45(28):1-2,35,3.基金项目
国家自然科学基金青年项目(21506235) （21506235）
安徽省自然科学基金面上项目(1508085MB38). （1508085MB38）
