安徽农业科学2017，Vol.45Issue(28)：3-6,10,5.
野火球总黄酮提取及抗氧化研究
Extraction of Total Flavonoids from Trifolium lupinaster L.and Its Antioxidant Activities
摘要
Abstract
[Objective] To optimize the extraction process for total flavonoids from Trifolium lupinaster L.and to study its antioxidant activities. [Method] Choosing the extraction rate of flavonoids as evaluation standard,the different methods,such as heating reflux method,soxhlet extration method and ultrasonic extraction,were comparatively analysed.Base on the single factor tests and the orthogonal experimental design,the best con-dition of ultrasonic extraction flavonoids was found and sifted.Furthermore,the DPPH radical scavenging capacity and FRAP total antioxidant ac-tivity tests were carried out to examine its antioxidant activities.[Result] The ultrasonic extraction was the best way to extract the total flavonoids from Trifolium lupinaster L.The optimal ultrasonic extract conditions were ethanol concentration of 50%,ultrasonic time of 75 min,solid-liquid ra-dio of 1:20 and ultrasonic power of 350 W.Under these optimized conditions, the yield of total flavonoids from Trifolium lupnaster L.was 1.686%.Additionally,by in vitro antioxidant assays,the total flavonoids extracted using ultrasonic extraction had a potent scavenging effect on DPPH free radical with IC50 value of (0.2077 ±0.0103) mg/mL and FRAP antioxidative capacity scavenging activity with value of (4.5610 ± 0.2280) mmol/g.[Conclusion] The ultrasonic extraction of total flavonoids was stable and feasible.Moreover,the extracts by this method exhibi-ted a better antioxidative activity.关键词
野火球/总黄酮/超声提取/正交试验/抗氧化Key words
Trifolium lupinaster L./Total flavonoids/Ultrasonic extraction/Orthogonal experiment/Antioxidant activity分类
医药卫生引用本文复制引用
姜守刚,袁颖洁,阮鑫,魏亮,张星垚,吴比,祖元刚..野火球总黄酮提取及抗氧化研究[J].安徽农业科学,2017,45(28):3-6,10,5.基金项目
国家林业局公益性行业专项(20140470102). （20140470102）
