北京师范大学学报（自然科学版）2017，Vol.53Issue(3)：294-300,7.DOI:10.16360/j.cnki.jbnuns.2017.03.009
大丽轮枝菌拮抗芽孢菌株的分离、鉴定及两株菌抑菌特性研究
Isolation and identification of antagonistic Bacillus spp.against Verticillium dahliae :the antibacterial properties of two strains
摘要
Abstract
Cotton Verticillium wilt is the most serious fungal disease in Chinese cotton production,screening for its control for stable and high yields is of great significance in China.We have isolated antagonistic Bacillus strains against Verticillium dahliae from healthy continuously-cropping cotton rhizosphere soil in Xinjiang.A total of 146 strains were obtained and 29 strains showed varied degrees of antagonism to Verticillium dahliae.Of these strains J-02 and J-29 belonged to Bacillus pumilus and the remainder are similar to B.subtilis.We found that the strongest inhibition of J-02 on Verticillium dahliae was in decline phase,J-15 in stable phase and inhibition was stable in the pH range from 5～9 for J-02 and 7～9 for J-15 respectively.Strain J-02 was found to be more resistant to heat,J-15 was sensitive to temperature,but there was still an inhibition zone after 100 ℃ treatment J-02 inhibited the germ tube of Verticillium dahilae elongates to form mycelium.J-15 caused mycelium of Verticillium dahliae to be deformed,and to fail to produce spores.In pot experiments,J 02 and J-15 showed a certain protective effect on cotton Verticillium wilt.In conclusion,strains J-02 and J-15 have potentials as biocontrol vectors against cotton Verticillium wilt.关键词
棉花黄萎病/大丽轮枝菌/拮抗作用/芽孢杆菌Key words
cotton Verticillium wilt/Verticillium dahliae/antagonism/Bacillus分类
农业科技引用本文复制引用
赵君洁,白若翔,韩生成,赵和平,赵惠新,曾卫军,李艳红,葛风伟,杜钰,袁琳琳,赵歉歉,王敏,谢红桃..大丽轮枝菌拮抗芽孢菌株的分离、鉴定及两株菌抑菌特性研究[J].北京师范大学学报（自然科学版）,2017,53(3):294-300,7.基金项目
新疆维吾尔自治区高校科研计划科学研究重点资助项目(20140705081400714) （20140705081400714）
新疆维吾尔自治区教育厅重点实验室“新疆特殊环境物种多样性应用与调控重点实验室”资助项目(XJTSWZ-2015-01) （XJTSWZ-2015-01）
新疆师范大学博士启动基金资助项目(XJNUBS1416) （XJNUBS1416）
抗性基因资源与分子发育北京市重点实验室开放基金资助项目(2015GD03) （2015GD03）
