动物营养学报2017，Vol.29Issue(10)：3604-3610,7.DOI:10.3969/j.issn.1006-267x.2017.10.022
金雀异黄素对雌性大鼠体内促性腺激素及胰岛素样生长因子表达的影响
Effect of Genistein on Expressions of Gonadotropins and Insulin?Like Growth Factors of Female Rats
摘要
Abstract
In order to study the effects of genistein ( GEN ) on expressions of gonadotropins and insulin?like growth factors in female rats, forty female SD rats [(200±20) g] were selected, and randomly divided into 5 groups with 8 rats per group, which were negative control ( NC) group, GEN low dose ( L) group, GEN middle dose ( M) group, GEN high dose ( H) group, positive control ( PC) group. Rats in NC group were in?tragastric administrated peanut oil ( other groups used it as solvent) , those in L, M and H groups were intra?gastric administrated GEN at 15, 30 and 60 mg/( kg BW·d) , and those in PC group were intragastric admin?istrated diethylstilbestrol at 0.5 mg/( kg BW·d) . The experiment lasted for 30 days. Serum contents of folli?cle?stimulating hormone ( FSH) , luteinizing hormone ( LH) , insulin?like growth factor?1 ( IGF?1) and insu?lin?like growth factor binding protein?1 ( IGFBP?1 ) were detected by enzyme linked immunosorbent assay ( ELISA) method. The mRNA expression levels of IGF?1 and IGFBP?1 in ovary tissue were detected by real?time quantitative PCR. The results showed as follows: compared with NC group, serum contents of FSH and LH in experimental groups tended to be increased, but the differences were not significant ( P>0.05) , which showed the same tendency with those in PC group; serum content of IGF?1 in experimental group was slightly decreased, but the difference was not significant ( P>0.05) , and that in PC group was significantly decreased (P<0.05); serum content of IGFBP?1 in experimental groups was significantly increased (P<0.05 or P<0.01), and that in PC group was significantly increased (P<0.05); mRNA expression levels of IGF?1 and IGFBP?1 in ovary tissue were increased, especially were significantly increased in M and H groups ( P<0.05) , which showed the same tendency with those in PC group. It is concluded that GEN can increase serum contents of FSH and LH, decrease serum IGF?1 content, and increase serum IGFBP?1 content, as well as increase mR?NA expression levels of IGFBP?1 and IGF?1 in ovary of rats. These indexes synergistically affect ovary, can promote follicle maturation and improve the function of ovary.关键词
金雀异黄素/卵泡刺激素/黄体生成素/胰岛素样生长因子-1/胰岛素样生长因子结合蛋白-1/雌性大鼠Key words
genistein/follicle⁃stimulating hormone/luteinizing hormone/insulin⁃like growth factor⁃1/insu⁃lin⁃like growth factor binding protein⁃1/female rat分类
农业科技引用本文复制引用
甄井龙,初晓丽,张涛,丛莎,迟晓星..金雀异黄素对雌性大鼠体内促性腺激素及胰岛素样生长因子表达的影响[J].动物营养学报,2017,29(10):3604-3610,7.基金项目
国家自然科学基金( 81673170 ) （ 81673170 ）
黑龙江省博士后科研启动基金( LBH?Q15112 ) （ LBH?Q15112 ）
黑龙江省研究生科研创新资金项目(YJSCX2016?Y42) （YJSCX2016?Y42）
黑龙江省高校农产品加工与质量安全创新团队课题(2014TD006) （2014TD006）
