动物营养学报2017，Vol.29Issue(10)：3627-3634,8.DOI:10.3969/j.issn.1006⁃267x.2017.10.025
植物乳杆菌-泰山松花粉多糖微胶囊对小鼠生长和肠道环境的影响
Preparation of Microcapsules Prepared with Lactobacillus plantarum and Taishan Pine Pollen Polysaccharide and Its Effects on Growth and Intestinal Environment of Mice
摘要
Abstract
Lactobacillus plantarum ( L. plantarum) , as a kind of microecological feed additive, was easy to be affected by adverse factors such as gastric acid, bile acid and digestive enzyme. Taishan pine pollen polysac?charide ( TPPPS) was a kind of water soluble plant polysaccharide, which was easy to be damaged by acid and enzyme. In order to protect the biological activity of L. plantarum and TPPPS and to explore whether they have synergistic effect, this experiment prepared microcapsules with L. plantarum and TPPPS as core materials. The microcapsule embedding process was optimized by orthogonal test, and the performance of the microcapsules prepared under the optimum conditions was determined. Forty?eight specific pathogen free ( SPF ) mice ( weighted about 20 g) were randomly divided into 4 groups with 12 mice per group. Mice in different groups were orally fed 1.0 mL of L. plantarum, L. plantarum+0.5% TPPPS, microcapsules and phosphate buffered saline ( PBS) ( control group) , respectively. The feeding trial lasted for 42 d. Every other week, three mice from each group were selected randomly and weighed. After autopsy, intestinal tissues were aseptically collect?ed and measured the changes of villi and crypts in the small intestine, and then the colonization of lactic acid bacteria and Escherichia coli were determined. The results showed as follows: 1) encapsulation efficiency of the microcapsules was 81. 6% and diameter was 84. 3 μm. Survival rate of L. plantarum was 62. 36% after 120 min in simulated gastric juice and 45.9% after 120 days storage at 37℃. 2) compared with control group, L. plantarum, L. plantarum +0.5% TPPPS and the microcapsules could increase body weight of mice at differ?ent degrees, increased the number of lactic acid bacteria in small intestine, reduced the number of Escherichia coli, improve development of small intestine villi and crypts, and the effects of the microcapsules were the best. The results indicate that probiotic effect of L. plantarum combined with TPPPS is better than that of L. plantarum alone, while the microcapsules prepared with the above combination can further improve their bene?ficial effects.关键词
植物乳杆菌/泰山松花粉多糖/微胶囊/小肠绒毛Key words
L. plantarum/Taishan pine pollen polysaccharide/microcapsules/small intestine villi分类
农业科技引用本文复制引用
马宁宁,裴宗飞,朱丽君,董雯雯,周建波,胡莉萍,魏凯,黄河,朱瑞良..植物乳杆菌-泰山松花粉多糖微胶囊对小鼠生长和肠道环境的影响[J].动物营养学报,2017,29(10):3627-3634,8.基金项目
山东省现代农业产业技术体系建设经费资助( SDAIT?10?06 ) （ SDAIT?10?06 ）
国家重点研发计划项目"牛羊重要疫病诊断与检测新技术研究"(2016YFD0500900) （2016YFD0500900）
