动物营养学报2017，Vol.29Issue(10)：3737-3749,13.DOI:10.3969/j.issn.1006-267x.2017.10.037
绿茶粉和绿茶多酚对犬的抗氧化作用
Antioxidant Effects of Green Tea Powder and Green Tea Polyphenols on Canines
摘要
Abstract
The present study was conducted to investigate the antioxidant effects of green tea powder and green tea polyphenols ( GPTs) on canines. Fifteen 5?month old canines were selected and randomly divided into three groups ( 5 canines in each group) . The canines in control group were fed a basal diet, the canines in green tea power group were fed the basal diet plus 1.00% green tea power, and the canines in GPTs group were fed the basal diet plus 0.25%GPTs. The feeding experiment lasted for 84 days. The results showed as follows: 1) compared with the initial body weight, the final body weight of control group, green tea power group and GTPs group was increased by 162%, 101% and 132%, respectively. The final body weight of green tea power group and GTPs group was significantly lower than that in control group ( P<0.05) , which was decreased by 61% and 30% compare with control group, respectively. On the 84th day of experiment, the activities of ser?um alanine transaminase ( ALT ) , aspartate aminotransferase ( AST ) and lactic dehydrogenase ( LDH ) in green tea power group and GTPs group were significantly or extremely significantly lower than those in control group (P<0.05 or P<0.01), the activities of serum glutathione peroxidase (GSH?Px) and superoxide dis?mutase ( SOD) were extremely significantly higher than those in control group ( P<0.01) , the content of ser?um malondialdehyde ( MDA) was extremely significantly lower than that in control group ( P<0.01) , and the relative mRNA expression levels of antioxidant genes [ glutamate cysteine ligase catalytic subunit ( GCLC ) , glutamate cysteine ligase subunit modification ( GCLM ) , heme oxygenase 1 ( HO?1 ) and catalase ( CAT ) ] and phase Ⅱ detoxification genes [ quinone oxidoreductase 1 ( NQO1 ) and glutathione S transferase M1 ( GSTM1) ] in liver were significantly higher than those in control group ( P<0. 01 ) . In summary, adding 1.00% green tea powder or 0.25% GTPs in the diet of canines have the effects of liver protection, antioxidant, activating phase Ⅱ detoxification gene expression and controlling body weight under the condition of the pres?ent study.关键词
犬/绿茶粉/绿茶多酚/肝功能/抗氧化基因/Ⅱ相解毒酶基因Key words
canines/green tea powder/green tea polyphenols/liver function/antioxidant genes/phase Ⅱdetoxification genes分类
农业科技引用本文复制引用
李玉,王一君,张梁,吴金节,宛晓春,陶焕青,张玉蝶,徐怡钟,郝宁,邹苏萍,冯士彬,王希春,周裔彬..绿茶粉和绿茶多酚对犬的抗氧化作用[J].动物营养学报,2017,29(10):3737-3749,13.基金项目
2016年度安徽省博士后研究人员科研活动经费资助项目(2016B117) （2016B117）
现代农业产业技术体系资助(CARS?19) （CARS?19）
茶叶化学与健康领军人才团队引进重大示范项目 （）
国家重点研发计划(2016YFD0501009) （2016YFD0501009）
