动物营养学报2017，Vol.29Issue(10)：3750-3760,11.DOI:10.3969/j.issn.1006⁃267x.2017.10.038
基于宏基因组学技术检测全株玉米青贮期间和暴露空气后的微生物多样性
Microbial Diversity of Whole?Plant Maize during Ensilage and after Air Exposure Analyzed by Metagenomics Technology
摘要
Abstract
The objective of this experiment was to analyze the fermentation quality and microbial diversity of whole?plant maize during ensilage and after air exposure, and to monitor the dynamic changes of microbial community composition. There were 3 sampling time points in this experiment including fermentation at the 5th day ( F5 group) , fermentation at the 40th day ( F40 group) and exposed air at the 3rd day after ensilage for 40 days ( A3 group) , and each sampling time point took 3 bags as 3 replicates. Microbial 16S rDNA V3 to V4 re?gion of whole?plant maize during ensilage and after air exposure were sequenced by metagenomics technology, and the microbial community composition and abundance of 3 groups were compared. Alpha diversity and prin?cipal component analysis were carried out to investigate the microbial diversity of whole?plant maize during en?silage and after air exposure. The results showed that the contents of pH, neutral detergent fiber ( NDF) and acid detergent fiber ( ADF) of the whole?plant maize after 40 days of silage fermentation were significantly de?creased ( P<0.05) , and the lactic acid content was significantly increased ( P<0.05) , which had a good fer?mentation quality and nutritional value, and short?time exposed air after 40 days for silage had no significantly effects on fermentation quality and nutritional value (P>0.05). A total of 122371 high?quality effective se?quences and 239 operational taxonomic units ( OTU) were obtained by Illumina Miseq sequencing platform, and totally 16 phyla, 163 genuses of bacteria were identified in samples of 3 groups. At the level of phylum, Firmicutes was dominant from beginning to end, and its abundance in F5, F40 and A3 groups was 57.57%, 74.65% and 78.82%, respectively, and showed a growth trend. Dominant species of the early stage for fer?mentation ( F5 group) , the late stage for fermentation ( F40) and the aerobic period ( A3 group) were Lacto?bacillus, which abundance was 49.78%, 64.46% and 45.34%, respectively, but the proportion of Sporolacto?bacillus in aerobic period was obvious increased by 28.46%. In conclusion, the natural fermentation of whole?plant maize silage significantly increases the abundance of Lactobacillus, which is beneficial to improve the fer?mentation quality of whole?plant maize silage, but exposed air at the 3rd day after ensilage has effect on the mi?crobial diversity of whole?plant maize silage. By Miseq high?throughput sequencing technology, we can fully understand the changes of microbial community composition and abundance of whole?plant maize during ensi?lage and after air exposure.关键词
青贮/全株玉米/宏基因组学/微生物多样性/发酵品质Key words
silage/whole⁃plant maize/metagenomics/microbial diversity/fermentation quality分类
农业科技引用本文复制引用
胡宗福,常杰,萨仁呼,王思珍,牛化欣..基于宏基因组学技术检测全株玉米青贮期间和暴露空气后的微生物多样性[J].动物营养学报,2017,29(10):3750-3760,11.基金项目
国家自然科学基金项目(31160474) （31160474）
优质肉牛生产核心技术研究与示范创业人才团队资助项目 （）
