动物营养学报2017，Vol.29Issue(10)：3761-3772,12.DOI:10.3969/j.issn.1006-267x.2017.10.039
长期饲喂不同蛋白质水平饲粮对猪脂肪代谢相关基因表达的影响
Effects of Long?Term Feeding Diets with Different Protein Levels on Gene Expressions Related to Lipid Metabolism of Pigs
摘要
Abstract
This experiment was conducted to study the effects of long?term feeding diets with different protein levels on gene expressions related to lipid metabolism of pigs. Eighteen cross?bred ( Duroc × Landrace × York?shire) piglets weaned 28 days of age were randomly assigned to 3 groups with 6 replicates per group and 1 pig?let per replicate. Piglets in control group [ ( high crude protein, HCP) group] were fed a diet based on the Na?tional Research Council (NRC) (2012) recommendation, those in experimental groups were fed diets with decreasing 3% [ ( medium crude protein, MCP) group] and 6% crude protein levels [ ( low crude protein, LCP) group], respectively, based on NRC (2012), and supplemented with 4 essential amino acids, which were lysine, methionine, threonine and tryptophane. The experiment lasted for 125 days. The results showed as follows:1) in liver, compared with HCP group, LCP group significantly decreased expressions of fatty acid synthesis genes including acetyl CoA carboxylase ( ACC) , fatty acid synthase ( FAS) , malicenzyme 1 ( ME1) and ankyrin 1 (ANK1) (P<0.05), and significantly increased expressions of fatty acid transport genes like peroxisome proliferator?activated receptor gamma ( PPARγ) and fatty acid binding proteins ( FABP ) ( P<0.05). However, there were no significant differences between MCP and HCP groups in hepatic expressions of fatty acid synthesis genes and fatty acid transport genes ( P>0.05) . Compared with HCP group, there were sig?nificant increases in hepatic expressions of fatty acid oxygenolysis genes like peroxisome proliferator?activated receptor alfa ( PPARα) and carnitine palmitoyltransferase ( CPT) in MCP and LCP groups ( P<0.05) . 2) In the longissimus dorsis muscle ( LDM) , the expressions of fatty acid synthesis genes like sterol regulatory ele?ment binding protein ( SREBP) , FAS and stearoyl CoA desaturase ( SCD) showed significantly higher in MCP group than those in the other two groups ( P<0.05) , but ACC and FABP displayed significantly lower expres?sions in LCP group than those in the other two groups ( P<0.05) . The expression of CPT gene and intramuscu?lar fat ( IMF) content of LDM were significantly lower in LCP and MCP groups compared to HCP group ( P<0.05) . 3) In liver and LDM, there were no significant differences in expressions of adipose triglyceride lipase (ATGL) and 2,4?dienoyl?CoA reductase (DECR) genes among three groups (P>0.05). In conclusion, de?creasing dietary crude protein level ( 3%) properly can promote expressions of fatty acid synthesis and transport genes, but has no significant effects on hepatic expressions of fatty acid synthesis genes; decreasing dietary crude protein level ( 3%) properly can decrease hepatic expressions of fatty acid oxygenolysis genes, but does not affect IMF content of LDM.关键词
饲粮/蛋白质/肥育猪/脂肪/代谢/基因Key words
diet/protein/finishing pigs/lipid/metabolism/gene分类
农业科技引用本文复制引用
田志梅,蒋宗勇,马现永,王丽,熊云霞,邱月琴,杨雪芬,高开国,温晓鹿,胡友军..长期饲喂不同蛋白质水平饲粮对猪脂肪代谢相关基因表达的影响[J].动物营养学报,2017,29(10):3761-3772,12.基金项目
国家 973 计划( 2013CB127301, 04 ) （ 2013CB127301, 04 ）
广东省现代农业产业技术体系( 2016LM1080 ) （ 2016LM1080 ）
广东省国际科技合作计划项目(2014A050503049) （2014A050503049）
国家现代农业产业体系(CAR?36) （CAR?36）
省级现代农业(畜禽健康养殖)产业技术研发中心建设项目 （畜禽健康养殖）
