动物营养学报2017，Vol.29Issue(10)：3819-3830,12.DOI:10.3969/j.issn.1006-267x.2017.10.046
大口黑鲈维生素A需求量
Dietary Vitamin A Requirement of Largemouth Bass ( Micropterus salmoides)
摘要
Abstract
This trial was conducted to estimate the dietary requirement of vitamin A in largemouth bass ( Mi?cropterus salmoides) . Five isonitrogenous ( crude protein was 48. 47%) and isoenergetic ( gross energy was 18.15 MJ/kg) diets, containing 415, 1406, 2755, 3501 and 4646 IU/kg vitamin A in diet, were formula?ted by adding vitamin A ( as retinyl acetate) 0, 1000, 2000, 3000 and 4000 IU/kg to a basal diet, respec?tively. Each diet was randomly assigned to triplicate tanks of 30 fish [initial body weight (52.75±0.12) g] for 49 d. The results showed that weight gain rate, specific growth rate, feed efficiency ratio and protein efficiency ratio occurred to a significant increasing tendency as dietary vitamin A level increased from 415 to 2755 IU/kg diet ( P<0.05) and tended to plateau in fish fed higher vitamin A levels. Liver vitamin A content, liver total superoxide dismutase activity, serum lysozyme activity, respiratory burst activity of head kidney leucocyte, se?rum complement activity and haemoglobin content were significantly increased with dietary vitamin A levels in?creasing from 415 to 3501 IU/kg, while malondialdehyde content in liver was significantly decreased ( P<0.05) , and the above indexes were slightly changed in fish fed higher dietary vitamin A levels. No significant differences in haematocrit and serum total protein content were found among groups ( P>0.05) . Based on bro?ken?line regression analysis for weight gain rate and vitamin A content in liver against dietary vitamin A level, largemouth bass require 2600 IU/kg vitamin A in diet for maximal weight gain, and 3550 IU/kg vitamin A in diet for maximal vitamin A deposition in liver, respectively. Based on analysis of variance for best non?specific immune index, the optimum requirement for dietary vitamin A is similar to that for maximal vitamin A deposi?tion in liver. It can be concluded from this study that the optimum dietary requirement of vitamin A for large?mouth bass is 2600 to 3550 IU/kg.关键词
大口黑鲈/维生素A需求量/生长性能/非特异性免疫Key words
largemouth bass ( Micropterus salmoides )/vitamin A requirement/growth performance/non⁃specific immune index分类
农业科技引用本文复制引用
连雪原,陈乃松,王孟乐,闫春为,丁桂涛..大口黑鲈维生素A需求量[J].动物营养学报,2017,29(10):3819-3830,12.基金项目
上海市科委项目(10320503100) （10320503100）
上海高校知识服务平台(ZF1206) （ZF1206）
