动物营养学报2017，Vol.29Issue(10)：3831-3836,6.DOI:10.3969/j.issn.1006-267x.2017.10.047
丁酸梭菌CBM01的碳、氮源优化及其对胃肠道耐受性的研究
Optimization of Carbon and Nitrogen Sources of Clostridium butyricum CBM01 and Its Tolerance to Gastrointerestinal Tract
摘要
Abstract
This experiment was conducted to investigate the optimal carbon and nitrogen source of Clostridium butyricum CBM01 and its tolerance to gastrointestinal tract. The reinforced clostridial medium ( RCM ) was used to optimize the carbon and nitrogen sources of Clostridium butyricum CBM01 with the method of control?ling variables, and the tolerance of Clostridium butyricum CBM01 to gastrointestinal tract was also determined. The results showed as follows:1) the best carbon source was glucose and the best nitrogen source was peptone for Clostridium butyricum CBM01; 2) the survival rates of of Clostridium butyricum CBM01 in the artificial gastric juice and artificial intestinal juice for 3 h were at 90.33% and 92.09%, respectively;3) the highest tol?erable concentration of Clostridium butyricum CBM01 to bile salt was 0.40%. Altogether, as Clostridium bu?tyricum CBM01 exerts great characteristics to tolerate gastrointestinal environments it can be considered as an i?deal potential probiotic strain for further researching.关键词
丁酸梭菌/碳源/氮源/耐胆盐/人工胃液/人工肠液Key words
Clostridium butyricum/carbon sources/nitrogen sources/tolerance of bile salt/artificial gastric juice/artificial intestinal juice分类
农业科技引用本文复制引用
刘磊,田亚男,倪学勤,曾东,潘康成,王鹤松..丁酸梭菌CBM01的碳、氮源优化及其对胃肠道耐受性的研究[J].动物营养学报,2017,29(10):3831-3836,6.基金项目
国家自然科学基金(31672318) （31672318）
