发光学报2017，Vol.38Issue(10)：1359-1365,7.DOI:10.3788/fgxb20173810.1359
糠醛缩壳寡糖席夫碱及Cu(Ⅱ)配合物与DNA之间的作用机制
DNA Binding Activity of Copper Complex with Schiff Base Derived from Oligochitosan and Furfuraldehyde
摘要
Abstract
Schiff base was synthesized by the reaction of oligochitosan with furfuraldehyde, and ac-ted it as ligand to prepare different molar Schiff base copper complexes which possessed electrochem-ical activity. The action mechanism of the complex and DNA was studied by spectrometry, viscosity measurement, cyclic voltammeiry and melting point experiment. The results indicate that the reac-tion of central Cu2+at glassy carbon electrodes is controlled by diffusion process mechanism. The in-tensity of the maximal absorption peaks of the complexes is weakened with the increasing of DNA concentration, and the oxidation peak current is decreased but no new redox peak appeared. At the same time, the viscosity and melting point of DNA are increased. There are intercalation action be-tween the complex and DNA, and DNA-binding ability order is FCOS-Cu11>FCOS-Cu13>FCOS-Cu31.关键词
席夫碱/配合物/结合DNAKey words
Schiff base/complex/DNA binding分类
化学化工引用本文复制引用
李小芳,冯小强,朱元成..糠醛缩壳寡糖席夫碱及Cu(Ⅱ)配合物与DNA之间的作用机制[J].发光学报,2017,38(10):1359-1365,7.基金项目
甘肃省青年科技基金计划(1606RJYE247) （1606RJYE247）
天水市科技支撑项目(2016 年) （2016 年）
甘肃省高等学校科学研究项目(2016B-071) （2016B-071）
天水师范学院中青年教师科研项目(TSA1508)资助Supported by Youth Science and Technology Fund Program of Gansu Province(1606RJYE247) （TSA1508）
Science and Technology Support Project of Tianshui City(2016) （2016）
Scientific Research Project Higher Education Institutions of Gansu Province (2016B-071) （2016B-071）
Re-search Project for Young and Middle-aged Teachers of Tianshui Normal University(TSA1508) （TSA1508）
