福建农林大学学报（自然科学版）2017，Vol.46Issue(5)：488-494,7.DOI:10.13323/j.cnki.j.fafu(nat.sci.).2017.05.002
小西葫芦黄花叶病毒辽宁分离物的鉴定与序列分析
Identification and sequence analysis of Zucchini yellow mosaic virus in Liaoning
摘要
Abstract
Cucurbita moschata samples showing yellow mosaic symptoms were collected from Shenyang and Gaizhou, Liaoning Prov-ince, and virions with a typical morphology of Potyvirus were observed by transmission electron microscope ( TEM) . The universal degenerate primers LegpotyF/LegpotyR was used for Potyviruses detection for 3 symptomatic leaf samples from Shenyang and Gaizhou by RT-PCR. Subsequently, the expected fragments were amplified, cloned and sequenced. Blast results showed that all 6 samples shared the highest identities with Zucchini yellow mosaic virus (ZYMV) (all above 99%). Based on specific primers ZYMV-F/ZYMV-R, 1.2 kb ZYMV fragment containing complete coat protein (CP) gene was obtained. Referring to the nucleotide sequence of CP, homology analysis results confirmed that two Liaoning isolates of ZYMV had the highest homology with Shanxi isolate ZYMV-[ CN:SX:Chrysanthemum:14] ( KP742962) , with identity being 99.1% and 99.4%. The phylogenetic tree results showed that they were also clustered with ZYMV-[ CN:SX:Chrysanthemum:14] in the same branch. Liaoning isolates of ZYMV was considered being most related to Shanxi isolates.关键词
小西葫芦黄花叶病毒/辽宁分离物/序列分析Key words
Zucchini yellow mosaic virus/Liaoning isolate/sequence analysis分类
农业科技引用本文复制引用
付晶晶,杨彩霞,韩彤,廖一鸣,李梁,李梦新,孙伟,王蒴..小西葫芦黄花叶病毒辽宁分离物的鉴定与序列分析[J].福建农林大学学报（自然科学版）,2017,46(5):488-494,7.基金项目
辽宁省自然科学基金(2015020806) （2015020806）
辽宁省教育厅一般项目(L2015362) （L2015362）
辽宁省博士科研启动基金(20121043) （20121043）
沈阳大学博士科研启动基金(20212365). （20212365）
