福建农林大学学报（自然科学版）2017，Vol.46Issue(5)：539-545,7.DOI:10.13323/j.cnki.j.fafu(nat.sci.).2017.05.010
植物ASK1-D3(MAX2)-D14蛋白复合体互作区特征与演化分析
Characterization and evolution analysis of the interaction interface of plant ASK1-D3( MAX2)-D14 complex
摘要
Abstract
ASK1-D3-D14 protein complex, as a part of E3 ubiquitin ligase, regulates plant morphogenesis and other physiological reactions. In order to characterize the interaction interface of the complex and reveal the evolutionary relationships of D3 orthologs, we searched and analyzed the protein sequences, and found that D3 genes showed different evolution processes among different spe-cies according to phylogenetic analysis. D3 protein contains F-box-like domain that interacts with ASK1 protein. Amino acid se-quences of F-box-likes in different proteins exhibit lower similarity, but their 3D structures share high similarity, implying that the similarities of their 3D structures are more important than sequences in terms of interaction. The amino acid sequences of the interac-tion interfaces between D3 and D14 orthologs are more similar, especially the amino acids sequence of hydrophobic areas between D3 and D14 proteins. These findings attribute to further study of D3 protein complex.关键词
ASK1-D3-D14/蛋白复合体/演化/互作Key words
ASK1-D3-D14/protein complex/evolution/interaction分类
农业科技引用本文复制引用
郑秀娟,翁群清,陈思雀,孙新立..植物ASK1-D3(MAX2)-D14蛋白复合体互作区特征与演化分析[J].福建农林大学学报（自然科学版）,2017,46(5):539-545,7.基金项目
高等学校博士学科点专项科研基金(30123515110006). （30123515110006）
