分析化学2017，Vol.45Issue(10)：1475-1481,7.DOI:10.11895/j.issn.0253-3820.171080
纳升液相色谱-质谱分析方法的重现性对尿液多肽组分析结果的影响
Effect of Reproducibility of Nano-liquid Chromatography-Mass Spectrometry on Analysis of Urinary Peptidomics
摘要
Abstract
As an extension of proteomics, peptidomics has been widely used in medical and biological researches. However, the effect of reproducibility of identification method on peptidomics is not yet clear. In this work, the urine sample of a healthy people was analyzed for seven times in parallel by nano-liquid chromatography-high-resolution tandem mass spectrometry. To illustrate the variability and stability among these experiments, the number of spectra, the utilization of total spectra, the number of identified peptides, the number of proteins, and the ionic strength and retention time of peptides were counted and compared. The average number of peptides was 208 and the standard deviation was 38. 7. After all of data were combined, 426 peptides belonging to 114 proteins were obtained, while only 109 peptides coming from 35 proteins were identified in each experiment, indicating that there were both an randomness and a relative stability for LC-MS analysis. Increasing the number of parallel experiments would expand the data set of peptidomics, but the rate of increase would decrease over 3 or more measurements. Compared with peptides, the results of peptidomics were more stable at protein level, indicating that proteins were more robustly peptidomics biomarker than the peptides.关键词
多肽组/尿液/重现性/偶然误差Key words
Peptidomics/Urine/Reproducibility/Random error引用本文复制引用
王勇,吴利,徐金玲,李水明,刘宁,姜亮..纳升液相色谱-质谱分析方法的重现性对尿液多肽组分析结果的影响[J].分析化学,2017,45(10):1475-1481,7.基金项目
本文系深圳市科技项目(JSGG20140703163838793)资助 （JSGG20140703163838793）
