华南农业大学学报2017，Vol.38Issue(5)：7-12,6.DOI:10.7671/j.issn.1001-411X.2017.05.002
谷胱甘肽对奥尼罗非鱼原代肝细胞增殖及生化功能的影响
Effects of glutathione on proliferation and biochemical function of primary hepatocyte in hybrid tilapia (Oreochromis niloticus×O.aureus)
摘要
Abstract
[Objective] To investigate the effects of glutathione (GSH) on proliferation and biochemical function of primary hepatocyte in hybrid tilapia (Oreochromis niloticus× O.aureus).[Method] Tilapia primary hepatocytes were cultured in cell culture medium with 0 or 10％ fetal calf serum for 48 h.GSH was then added into the two kinds of cell culture medium to the final concentration of 0,30,100,300 or 900 mg·L-1 respectively.Each concentration group had six replicates.The proliferation status of the primary hepatocyte,contents of insulin-like growth factor 1 (IGF-1),albumin and H2O2,as well as glutamate-oxaloacetate transaminase (GOT) and γ-glutamyl transpeptidase (γ-GT) activities of tilapia were detected after cultured for 24,48 and 72 h,respectively.[Result] GSH significantly increased the proliferation of primary hepatocyte,the contents of IGF-1 and albumin,and γ-GT activity in supernatant,while reduced H2O2 content and GOT activity(P＜0.05).Such effects of GSH were more evident in the group without fetal calf serum compared to the group with 10％ fetal calf serum.[Conclusion] GSH could enhance the proliferation of primary hepatocyte and has the function of protecting liver in hybrid tilapia.关键词
谷胱甘肽/奥尼罗非鱼/原代肝细胞/增殖/生化功能Key words
glutathione/Oreochromis niloticus×O.aureus/primary hepatocyte/proliferation/biochemical function分类
农业科技引用本文复制引用
陈标,焦彩红,朱选,潘庆..谷胱甘肽对奥尼罗非鱼原代肝细胞增殖及生化功能的影响[J].华南农业大学学报,2017,38(5):7-12,6.基金项目
广东省科技厅研发与产业化项目(2013B090500028) （2013B090500028）
广州市科技计划项目(2014YZ-00208) （2014YZ-00208）
