华南农业大学学报2017，Vol.38Issue(5)：13-18,6.DOI:10.7671/j.issn.1001-411X.2017.05.003
3株H1N1亚型猪流感病毒的HA基因遗传信息及抗原特异性分析
Analysis of genetic information in HA genes and antigen specificities of three strains of H1N1 swine influenza virus
摘要
Abstract
[Objective] To study the genetic information in HA genes and antigen specificities of SWSS1,SWL6 and Pdm091057 strains which respectively belongs to Eurasian avaian-like,classical and Pdm09H1N1 branches of H1N1 swine influenza viruses (SIV),and provide a basis for functional study on antigenic epitopes of HA gene as well as prevention and control of influenza virus.[Method] We compared the genetic information in HA gene fragments of three H1N 1 subtypes,SWSS 1,SWL6 and Pdm091057.Inactivated SIV vaccines were prepared and each type of vaccine was used to immune three New Zealand white rabbit.The hemagglutination inhibition (HI) reaction was used to detect antibody titers.[Result] The amino acid sequences of HA gene fragments had 69.4％-89.1％ similarities among three SIV strains.There were variations in antigenic epitopes of HA genes of the three strains.The average HI antibody titers were all above 1 280 for three strains after the second immunization,and the average HI antibody titer for SWSS1 reached 2 560.SWSS1 had no serological cross reaction with SWL6 or Pdm091057 strain,while SWL6 and Pdm091057 strains had low degree of serological cross reaction.[Conclusion] Three SIV strains have differences in antigenic epitopes of HA gene,which may be the reason for the low degree of serological cross reaction among strains.All three strains have good immunogenicity,and can be used as candidate vaccine strains.关键词
猪流感病毒/H1N1/遗传信息/抗原表位/血凝抑制/抗体滴度/HA基因Key words
swine influence virus/H1N1/genetic information/antigenic epitope/hemagglutination inhibition/antibody titer/HA gene分类
农业科技引用本文复制引用
秦锋,付新亮,曹振鹏,张桂红,蔡孟楷,张伟东,张京,方博,黄俊明,卜德新,罗勇峰,马骏..3株H1N1亚型猪流感病毒的HA基因遗传信息及抗原特异性分析[J].华南农业大学学报,2017,38(5):13-18,6.基金项目
国家重点研发计划(2016YFD0500707) （2016YFD0500707）
国家生猪产业技术体系专项(CARS-36) （CARS-36）
广东省科技计划(2013B020202002) （2013B020202002）
广东省条财处项目(2012B060400015) （2012B060400015）
广东省高等学校优秀青年教师培养计划(YQ201530) （YQ201530）
