华南农业大学学报2017，Vol.38Issue(5)：67-73,7.DOI:10.7671/j.issn.1001-411X.2017.05.012
全二维气相色谱-飞行时间质谱分析紫藤种子提取物及其杀蚜活性研究
GC×GC-TOF/MS analysis of the seed extract of Wisteria sinensis and the insecticidal activity
摘要
Abstract
[Objective] To analyze the chemical composition,insecticidal and antioxidant activities of the seed extract of Wisteria sinensis,and provide a basis for developing botanical pesticide from W.sinensis germplasm resources.[Method] The seed extract of W.sinensis was analyzed by comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC×GC-TOF/MS).The toxicity against Aphis gossypii was determined by topical application bioassay.The antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method.[Result] A total of 61 components were identified from the seed extract of W.sinensis,mainly including esters,alkane,arene,etc.The constituents with higher than 9％ relative content were p-xylene (14.33％),undecane (11.89％),1,4-diethyl-benzene (11.02％) and decane (9.54％).The seed extract of W.sinensis showed insecticidal activity against A.gossypii with the LCs0 value of 193.22 mg·L-1 after 24 h treatment,and also showed antioxidant activity with the IC50 value of 4.15 g·L-1 against DPPH.[Conclusion] The seed extract of W.sinensis has strong insecticidal activity,and has the potential to be developed into a natural insecticide for controlling A.gossypii.关键词
紫藤/种子提取物/全二维气相色谱-飞行时间质谱/化学成分/触杀活性/棉蚜/抗氧化活性Key words
Wisteria sinensis/seed extract/GC×GC-TOF/MS/chemical composition/contact toxicity/Aphis gossypii/antioxidant activity分类
农业科技引用本文复制引用
曹先爽,王进,宋丽,姜浩,姚曦,汤锋,岳永德..全二维气相色谱-飞行时间质谱分析紫藤种子提取物及其杀蚜活性研究[J].华南农业大学学报,2017,38(5):67-73,7.基金项目
“十三五”国家重点研发计划(2016YFD0600801) （2016YFD0600801）
国际竹藤中心基本科研业务费专项(1632014009) （1632014009）
