湖南农业大学学报（自然科学版）2017，Vol.43Issue(5)：504-511,8.DOI:10.13331/j.cnki.jhau.2017.05.007
南高丛蓝莓快繁体系的建立
Rapid propagation system establishment for southern highbush blueberry
摘要
Abstract
In order to figure out the best experimental conditions and to establish a complete and efficient system for rapid propagation of blueberry, a series of experiments were conducted on explant sterilization, buds induction, subculture of tissue-cultured seedlings, rooting and transplanting of tissue-cultured seedlings by employed twigs of 'South Golden', a variety of southern highbush blueberries, stems with axillary buds were used as explants. The results indicated that the explants soaked in 5% NaClO solution for 10 min, then in 0.1% HgCl2 solution for 7 min displayed the best effect in disinfection. Modified WPM with 4.0 mg/L ZT was the best medium for inducing buds, and the multiplication coefficient reached to 3.86, the average height of buds was 19.4 mm; modified WPM with 1.0 mg/L ZT was the best subculture medium, the multiplication coefficient was 1.82 and the average height of seedlings on this medium reached to 44.7 mm; modified 1/4WPM with 0.5 mg/L IBA and 1.0 mg/L NAA was considered as the best medium for inducing roots of tissue-cultured seedlings; vermiculite mixed with peat soil (the proportion of them was 1:1) was the most appropriate substrate for transplanting and cultivating tissue-cultured seedling, the survival rate reached up to 96.95%.关键词
南高丛蓝莓/诱导培养/继代培养/生根培养/植物激素Key words
southern highbush blueberry/induced culture/subculture/rooting culture/phytohormone分类
农业科技引用本文复制引用
金如意,郭崇炎,王志龙,陈秋红,胡方志..南高丛蓝莓快繁体系的建立[J].湖南农业大学学报（自然科学版）,2017,43(5):504-511,8.基金项目
教育部创新团队发展计划项目(IRT1239) （IRT1239）
