基础医学与临床2017，Vol.37Issue(10)：1368-1372,5.
5-氟尿嘧啶通过促进miR-22表达抑制肝癌细胞增殖
5-Fluorouracil suppresses human hepatocellular carcinoma cell growth via promoting miR-22 expression
摘要
Abstract
Objective To investigate the impact of 5-fluorouracil (5-FU) on miR-22 expression in human hepato-cellular carcinoma (HCC) cell lines and to elucidate the molecular mechanism of 5-fluorouracil for HCC chemo-therapy. Methods Real-time PCR analysis was conducted to determine the expression levels of miR-22 in HCC tissue specimens and HCC cell lines. The expression of miR-22 and pri-miR-22 (primary miR-22) was evaluated in HepG2 and Huh7 cells with 5-FU treatment by using real-time PCR and we also performed Western blot analysis to detect the protein level of HDAC4 in HCC cells with the same treatment. A rescue assay was employed by using 5-FU treatment in combination with miR-22 inhibitor(Anti-22) to further investigate the correlation among 5-FU, miR-22,and HCC cell growth. Results miR-22 expression depicted a significant downregulation in HCC tissues and cell lines (P<0.01). 5-FU treatment led to an augment of miR-22 expression in HepG2 and Huh7 cells(P<0.001) and resulted in a decrease of HDAC4 protein levels, which was verified as a direct target of miR-22 in HCC cells (P<0.01). Conclusions 5-FU has suppressive effect on HCC growth which could be potentially ex-plained by miR-22-mediated HDAC4 axis.关键词
miR-22/5氟尿嘧啶/肝细胞癌/化疗Key words
miR-22/5-fluorouracil/HCC/chemotherapy分类
医药卫生引用本文复制引用
孙蒙清,杜永星,李宗泽,由磊,舒红,刘子文..5-氟尿嘧啶通过促进miR-22表达抑制肝癌细胞增殖[J].基础医学与临床,2017,37(10):1368-1372,5.基金项目
国家自然科学基金(81272767) （81272767）
