南京林业大学学报（自然科学版）2017，Vol.41Issue(4)：6-12,7.DOI:10.3969/j.issn.1000-2006.201610064
青钱柳叶多糖不同组分体外降血糖及抗氧化活性研究
Hypoglycemic and antioxidant activity of various polysaccharides from Cyclocarya paliurus leaves in vitro
摘要
Abstract
[Objective] In order to evaluate the hypoglycemic and antioxidant activity of polysaccharides from Cyclocarya paliurus leaves in vitro.The activity of refined polysaccharides and precipitation fractionations of purified C.paliurus polysaccharide obtained from different concentrations of ethanol were compared in this study.[Method] The activity of inhibiting α-glucosidase was used to evaluate the hypoglycemic effect in vitro.The ability of total antioxidant,scavenging DPPH and (·OH) radicals and chelating Fe2+ were used to evaluate the antioxidant capacity.[Result]The results showed that refined polysaccharides of C.paliurus and different precipitation fractionations all showed a high inhibition against α-glucosidase,and showed a dose-effect relationship.With the ethanol concentration increasing,the fractions obtained showed a higher inhibition activity.Refined polysaccharides of C.paliurus and different precipitation fractionations all showed a good antioxidant activity.Among different precipitation fractionations,the abilities of scavenging DPPH and total antioxidant were increased with the ethanol concentration increasing.In contrast,the abilities of scavenging (· OH)radicals and chelating Fe2+ were decreased.[Conclusion] Different C.paliurus polysaccharides have good in vitro hypoglycemic and antioxidant activity,which can be used for further manufacture and expoliting.关键词
青钱柳/多糖/降血糖/抗氧化Key words
Cyclocarya paliurus/polysaccharide/hypoglycemic effect/antioxidant activity分类
轻工纺织引用本文复制引用
徐静珠,吴彩娥,应瑞峰,李婷婷,范龚健,方升佐..青钱柳叶多糖不同组分体外降血糖及抗氧化活性研究[J].南京林业大学学报（自然科学版）,2017,41(4):6-12,7.基金项目
国家自然科学基金项目(31270673) （31270673）
江苏高校优势学科建设工程资助项目(PAPD) （PAPD）
