南京林业大学学报（自然科学版）2017，Vol.41Issue(4)：30-36,7.DOI:10.3969/j.issn.1000-2006.201609007
马尾松PmCBL3基因的克隆及其表达分析
Cloning and expression analysis of PmCBL 3 from Pinus massoniana
摘要
Abstract
[Objective] CBLs participates in plants response to abiotic stress.The objectives of this study were to investigate biological function of CBL,and to provide a candidate gene and theoretical basis for the drought tolerant breeding for Pinus massoniana,by cloning the CBL from P.massoniana,and analyzing its sequence features,evolutionary relationships and expression characteristics.[Method] The full-length cDNA of CBL had been isolated from P.massoniana by RT-PCR and RACE,and bioinformatics analysis was conducted to analyze the properties and phylogenetic tree.In addition,the expression profile of CBL was analyzed by qRT-PCR under drought stress.[Result] A full-length cDNA,designated PmCBL3,was obtained.Sequence analysis revealed that PmCBL3 was 1 035 bp in length,including a 681 bp open reading frame that encodes 226 amino acids.PmCBL3 comprised four EF-Hand functional domains,and the distance between the EF-hand motifs was conservative.Evolutionary analysis further revealed that PmCBL3 had the closest relationship with Picea sitchensis,and CBL exhibited 62％-98％ homologies with other plants.qRT-PCR results showed that PmCBL3 expression was the highest in the roots,then was in the stems and leaves.PmCBL3 expression was in the peak at 15 d after treatment,but decreased with drought stress aggravation.[Conclusion] The results indicated that PmCBL3 belonged to the member of CBLs family genes,and had the closest relationship with CBL of P.sitchensis.PmCBL3 responded drought stress,which suggested that it was involved in drought stress regulation关键词
马尾松/PmCBL3/基因克隆/表达分析Key words
Pinus massoniana/PmCBL3/gene cloning/expression analysis分类
农业科技引用本文复制引用
蔡琼,丁贵杰,丁波,张仁波,杜明凤..马尾松PmCBL3基因的克隆及其表达分析[J].南京林业大学学报（自然科学版）,2017,41(4):30-36,7.基金项目
国家自然科学基金项目(31260183) （31260183）
“十三五”国家科技支撑计划(2015BAD09B0102) （2015BAD09B0102）
贵州省重大专项项目(黔科合重大专项字[2012]6001号) （黔科合重大专项字[2012]6001号）
贵州省人才基地建设项目(黔人领发[2009]9号) （黔人领发[2009]9号）
