南京林业大学学报（自然科学版）2017，Vol.41Issue(4)：37-41,5.DOI:10.3969/j.issn.1000-2006.201601057
两株松材线虫拮抗细菌的筛选和鉴定
The screening and identification of two bacterial strains with nematicidal activity against Bursaphelenchus xylophilus
摘要
Abstract
[Objective] The aim of the present study was to screen bacteria with nematicidal activity against the pine wood nematode (Bursaphelenchus xylophilus).[Method] A total of 137 bacteria strains,which were isolated from the stems of pine trees in Nanjing,Luoyang and Shanghai,China,were assessed for nematicidal activity by immersion test with cultural filtrate and buffer suspension.[Result] Cultural filtrates of three strains had stronger nematicidal activity than the other strains,and nematodes treated with these strains reached 100％ mortality after 48 h and even exhibited decomposition when treated with the cultural filtrate of strain LYMC-3.The cultural filtrates of three strains,which were diluted 2,4 and 10 times still showed high nematicidal activity.Nematicidal activity decreased with increasing dilution,and when nematodes were treated with 10× diluted filtrates for 48 h,the greatest observed mortality was 94.7％ withstrain LYMC-3.Moreover,the buffer suspension of strain NJSZ-13 had strong nematicidal activity against B.xylophilus,and 81.5％ nematode mortality was achieved after 48 h treatment with 105 cfu/mL buffer suspension of strain NJSZ-13.Based on morphology,physiological and biochemical characteristics,Biolog system identification,16SrDNA sequences,and a phylogenetic tree,the strains LYMC-3 and NJSZ-13 were identified as Bacillus pumilus and B.cereus,respectively.[Conclusion] Two strains of bacteria LYMC-3 and NJSZ-13 with high nematicidal activity which were isolated from the stems of pine trees could provide potential solution for biological control of Bursaphelenchus xylophilus.关键词
松材线虫/拮抗细菌/短小芽孢杆菌/蜡样芽孢杆菌Key words
Bursaphelenchus xylophilus/antagonistic bacteria/Bacillus pumilus/Bacillus cereus分类
农业科技引用本文复制引用
李亮亮,谈家金,陈凤毛..两株松材线虫拮抗细菌的筛选和鉴定[J].南京林业大学学报（自然科学版）,2017,41(4):37-41,5.基金项目
江苏省高校自然科学基金重大项目(14KJA220002) （14KJA220002）
江苏省普通高校研究生科研创新计划项目(KYLX16_0859) （KYLX16_0859）
国家自然科学基金项目(31370643) （31370643）
