南京林业大学学报（自然科学版）2017，Vol.41Issue(4)：191-196,6.DOI:10.3969/j.issn.1000-2006.201611006
柳杉无性系指纹图谱的构建及遗传多样性分析
Analysis of genetic diversity and construction of DNA fingerprinting of clones in Cryptomeria fortune
摘要
Abstract
[Objective] Molecular fingerprinting and genetic diversity analysis are important for protecting and managing superior germplasm resources,as well as for identifying superior clones.[Method] In the present study,11 SSR primer pairs were used to assess the genetic diversity and DNA fingerprints of 89 C.fortunei clones.[Result] A total of 53 putative alleles were detected from 11 pairs of primers,with a mean of five putative alleles per locus,ranging from three to six.The number of effective alleles(Ne)ranged from 1.887 0 to 4.295 6,with a mean of 3.041 1,and the Shannon's information index ranged from 0.774 9 to 1.556 3,with a mean of 1.208 1.These findings indicate that SSR markers in C.fortunei exist a relative high polymorphism.Furthermore,according to the characteristics of the SSR markers and primer sequences,the present (recorded as 1) and absent (recorded as 0) collected from amplification by SSR markers were transferred into genetic types.By combining different genetic types code,we established DNA fingerprints of 89 C.fortunei clones,and attached a 22-digit fingerprint number to each clone.The 89 clones were also separated into five clusters based on genetic distance.[Conclusion] SSR markers are suitable for analyzing and identifying the genetic diversity of C.fortune,and C.fortune shows moderate to high genetic diversity.关键词
SSR标记/柳杉无性系/遗传多样性/指纹图谱Key words
SSR marker/Cryptomeria fortunei/clones/genetic diversity/DNA fingerprinting分类
农业科技引用本文复制引用
骆鹏,曹玉婷,莫家兴,翁怀峰,施季森,徐进..柳杉无性系指纹图谱的构建及遗传多样性分析[J].南京林业大学学报（自然科学版）,2017,41(4):191-196,6.基金项目
国家林业公益性行业科研专项项目(201304104) （201304104）
江苏省“六大人才高峰”项目(2014-NY-013) （2014-NY-013）
江苏高校优势学科建设工程资助项目(PARD) （PARD）
福建省林木种苗科技攻关五期项目 （）
