南京林业大学学报（自然科学版）2017，Vol.41Issue(4)：209-214,6.DOI:10.3969/j.issn.1000-2006.201611044
海拔对黄山松阔叶混交林土壤微生物功能多样性的影响
Soil microbial characteristics of Pinus taiwanensis and broad-leaved mixed forest at different altitudes in Fengyang Mountain
摘要
Abstract
[Objective] To study the soil physicochemical properties and the use of carbon sources by microorganisms,we investigated the effects of altitude change on soil microbial functional diversity in the Pinus taiwanensis broad-leaved mixed forest of Fengyang Mountain.[Method]Using the main distribution range from 1 000 m to 1 800 m of Pinus taiwanensis in Fengyang Mountain as the standard,we chose three altitude gradients,respectively:medium altitude (1200m),middle-high altitude (1 500 m),and high altitude (1 800 m).We selected the best site in each altitudinal gradient drainage,on a sunny,gentle gradient slope,with slow,moderate growth in three standard plots (20 m×20 m).We applied the method of Biolog Eco Plate to study the carbon utilization of soil microorganisms in this type of forest at different altitude gradients.We used non-metric multidimensional scaling (NMDS) to order and further analyze the use of various carbon sources by soil microbes.[Result] ① The soil microbial activity was the highest at the middle-high altitude,whereas the soil microbial diversity index did not change significantly with altitude (P＞0.05).② In the study area,the main types of carbon source used were carbohydrates (CH),amino acids (AA),and polymers (PM).The utilization rate of phenolic acids (PA) and amine (AM) carbon sources varied with altitude.There were significant differences in the utilization of the carbon source carboxylic acid (CA) at different altitudes.The NMDS ordination showed that the use of six carbon sources varied with the change in altitude (P＜0.05).③The soil nutrient content was the highest in the middle-high altitude areas,and the redundancy analysis showed that there was a strong correlation between the physicochemical properties of soil and the utilization of carbon.[Conclusion] In the middle-high altitude areas,soil microbes have a strong effect on the utilization of various carbon sources,and the change in soil physical and chemical properties is an important factor affecting the utilization of carbon sources.关键词
黄山松阔叶混交林/土壤微生物功能多样性/Biolog Eco微平板技术/海拔Key words
Pinus taiwanensis broad-leaved mixed forest/soil microbial functional diversity/Biolog Eco plate/altitude分类
农业科技引用本文复制引用
孟苗婧,张金池,郭晓平,吴家森,赵有朋,叶立新,刘胜龙..海拔对黄山松阔叶混交林土壤微生物功能多样性的影响[J].南京林业大学学报（自然科学版）,2017,41(4):209-214,6.基金项目
国家林业公益性行业科研专项项目(201504406) （201504406）
江苏高校优势学科建设工程资助项目(PAPD) （PAPD）
