日用化学工业2017，Vol.47Issue(9)：517-521,5.DOI:10.13218/j.cnki.csdc.2017.09.008
天然椰子油提取物对H2 O2诱导的HSF细胞氧化损伤的保护作用
Protective effects of virgin coconut oil extracts on oxidative damage of human skin fibroblasts cells induced by H2 O2
摘要
Abstract
The human skin fibroblasts (HSF)cell morphology was observed by inverted biological microscope, the cell viability was detected by MTT method,and the intracellular ROS level,the intracellular total antioxidation capacity and the activity of antioxidation enzyme (SOD,CAT and GSH-Px)were examined for investigating the protective effects of virgin coconut oil extracts on oxidative damage of HSF cells induced by H2 O2 . Results show that the virgin coconut oil extracts could protect HSF cells from oxidative damage induced by H2 O2 at mass concentration range of 12. 5 to 50 mg/L with a concentration dependent manner. Virgin coconut oil extracts could inhibit the cell morphological damage induced by H2 O2 and improve the survival rate of the cells. In addition,virgin coconut oil extracts could protect HSF cells from oxidative damage induced by oxidative stress through decreasing the intracellular ROS level,increasing intracellular total antioxidant capacity and the enzyme activity of SOD,CAT and GSH-Px.关键词
化妆品原料/天然椰子油/HSF细胞/抗氧化/抗衰老Key words
cosmetic ingredient/virgin coconut oil/HSF cells/antioxidant/anti-aging分类
化学工程引用本文复制引用
宋菲,王挥,唐敏敏,沈晓君,张玉锋,邓福明..天然椰子油提取物对H2 O2诱导的HSF细胞氧化损伤的保护作用[J].日用化学工业,2017,47(9):517-521,5.基金项目
海南省自然科学基金资助项目(20163143) （20163143）
海南省重点研发计划资助项目(ZDYF2017129) （ZDYF2017129）
中国热带农业科学院基本科研业务费专项资金资助项目(1630152017011) （1630152017011）
