色谱2017，Vol.35Issue(10)：1037-1041,5.DOI:10.3724/SP.J.1123.2017.06013
基于高分辨质谱技术的婴幼儿食品中过敏原蛋白质的高灵敏检测
High-sensitive detection of multiple allergenic proteins in infant food with high-resolution mass spectrometry
摘要
Abstract
A novel method of the simultaneous detection of multiple kinds of allergenic proteins in infant food with parallel reaction monitoring (PRM)mode using liquid chromatography-tan-dem mass spectrometry (LC-MS/MS)was established. In this method,unique peptides with good stability and high sensibility were used to quantify the corresponding allergenic proteins. Furthermore,multiple kinds of allergenic proteins are inspected simultaneously with high sensi-tivity. In addition,such method was successfully used for the detection of multiple allergenic proteins in infant food. As for the sample preparation for infant food,compared with the tradi-tional acetone precipitation strategy,the protein extraction efficiency and capacity of resisting disturbance are both higher with in-situ filter-aided sample pretreatment (i-FASP)method. All allergenic proteins gave a good linear response with the correlation coefficients (R2 )≥0. 99, and the largest concentration range of the allergenic proteins could be four orders of magni-tude,and the lowest detection limit was 0. 028 mg/L,which was better than that reported in references. Finally, the method was conveniently used to detect the allergens from four imported infant food real samples. All the results demonstrate that this novel strategy is of great significance for providing a rapid and reliable analytical technique for allergen proteomics.关键词
平行反应监测/液相色谱-串联质谱/过敏原蛋白质/婴幼儿食品Key words
parallel reaction monitoring (PRM )/liquid chromatography-tandem mass spectrometry (LC-MS/MS)/allergenic protein/infant food分类
化学化工引用本文复制引用
吴慈,张丽华,陈溪,刘健慧,张晓林,薛伟锋,梁振,刘梦遥,崔妍,黄大亮..基于高分辨质谱技术的婴幼儿食品中过敏原蛋白质的高灵敏检测[J].色谱,2017,35(10):1037-1041,5.基金项目
中国科学院分离分析化学重点实验室开放基金项目(KL1606) （KL1606）
国家质量监督检验检疫总局科技计划项目(2015IK343).Open Fund Supported by Key Laboratory of Separation Sciences for Analytical Chemistry,Chinese Academy of Sciences (No. KL1606) （2015IK343）
Science and Technology Project of State General Administration of the People's Republic of China for Quality Supervision and Inspection and Quarantine (No. 2015IK343). （No. 2015IK343）
