CHEUK Man-Yung 1LO Yun-Chuen 1POON Wing-Tat1
作者信息
- 1. Department of Pathology,Pamela Youde Nethersole Eastern Hospital,Hong Kong 999077,China
- 折叠
摘要
Abstract
The measurement of urine catecholamine and metanephrine concentrations is important for biochemical screening and diagnosis of pheochromocytoma. The goal of this work was to develop a simple liquid chromatography-tandem mass spectrometry (LC-MS/MS ) method for determining catecholamines and metanephrines in urine to replace an existing liquid chromatographic method using electrochemical detection. Urine samples were prepared using Oasis weak-cation-exchange cartridges. The eluate was analyzed on an Agi-lent ZORBAX Eclipse Plus Phenyl-Hexyl column in 3 min. Adrenaline,noradrenaline,dopamine,metanephrine, normetanephrine,and their deuterated internal standards were monitored in positive electrospray ionization mode by multiple reaction monitoring (MRM ). No evidence of ion suppression was observed. The assay was linear up to 5 μmol/L for adrenaline,5 μmol/L for noradrenaline,6. 1 μmol/L for dopamine,5. 6 μmol/L for metanephrine,and 34. 6 μmol/L for normetanephrine,with lower limits of quantification of 5,5,12,6 and 7 nmol/L,respectively. The intra-day and inter-day precisions for all analytes ranged from 0. 59% to 4. 64% and 1. 98% to 4. 80%,respectively. External quality assurance samples were assayed and showed excellent agree-ment with the target values. This simple method provides an improved assay for determining urine cate-cholamines and metanephrines.关键词
liquid chromatography-tandem mass spectrometry (LC-MS/MS)/metanephrines/catecholamines/pheochromocytoma/urineKey words
liquid chromatography-tandem mass spectrometry (LC-MS/MS)/metanephrines/catecholamines/pheochromocytoma/urine分类
化学化工
