水生态学杂志2017，Vol.38Issue(5)：65-71,7.DOI:10.15928/j.1674-3075.2017.05.009
枯草芽孢杆菌培育生物絮团对池塘水体浮游生物的影响
Effect of a Bacillus subtilis Biofloc on Plankton in Pond Water
摘要
Abstract
Biofloc is an advanced technology in aquaculture and can effectively reduce excessive nitrogen in water, improve the immunity of aquatic animals and provide natural food for some fish species and shrimp. In this study, a biological flocculation test was conducted to evaluate the effects of biofloc on the species number and quantity of plankton in pond water, aiming to provide a reference for treatment of pond culture wastewater and to extend exist-ing research on biofloc technology. From May to August, 2015, a biological flocculation test was carried out in an aquarium (100 cm × 60 cm × 50 cm) with 250 L of pond water, using glucose as the carbon source and Bacillus subtilis as the experimental strain. Four groups were set for the experiment:a control group;Treatment I, supple-mented with glucose; Treatment Ⅱ, supplemented with both glucose and Bacillus subtilis; Treatment Ⅲ, supple-mented with Bacillus subtilis. The quantity of glucose added in Treatments Ⅰ and Ⅱ resulted in a carbon-nitrogen ra-tio of 20 : 1 and Bacillus subtilis was added to give 109 CFU/L in Treatments Ⅱ and Ⅲ. During the 40 day test, plankton and biofloc were monitored at five day intervals. Biofloc formation was enhanced by the addition of glucose to culture water. The addition of glucose and Bacillus subtilis shortened the formation time of a mature biofloc by a-bout five days, compared to addition of glucose alone. In Treatments I and II, the formation of a mature biofloc both peaked on day 15. Early in the test, phytoplankton abundance in Treatments Ⅰ and Ⅱ was significantly higher than in Treatment Ⅲ and the control, but later the abundance of Microcystis, Lyngbya and Platymonas, Gonium and Volvax in Treatments I and Ⅱ was significantly lower than those in Treatment Ⅲ and control. These results indi-cate that the formation of a biofloc initially increases phytoplankton abundance and then later inhibits the growth of Microcystis, Lyngbya and Platymonas, Gonium and Volvax. The abundance of rotifers, cladocera and copepods in Treatments Ⅰ and Ⅱ was also significantly higher than in Treatment Ⅲ and control late in the experimental period, suggesting that the biofloc promotes the growth of rotifers, cladocera and copepods.关键词
枯草芽孢杆菌/生物絮团/浮游植物/浮游动物Key words
Bacillus subtilis/biofloc/phytoplankton/zooplankton分类
生物科学引用本文复制引用
聂伟,刘立鹤,刘稳,付坦,周哲,吴鹏飞..枯草芽孢杆菌培育生物絮团对池塘水体浮游生物的影响[J].水生态学杂志,2017,38(5):65-71,7.基金项目
江苏省级工业和信息产业转型专项(编号2015187 ) （编号2015187 ）
湖北省教育厅教改项目(2016337). （2016337）
