中国生态农业学报2017，Vol.25Issue(10)：1463-1474,12.DOI:10.13930/j.cnki.cjea.170335
施氮量对玉米/大豆套作系统土壤微生物数量及土壤酶活性的影响
Effects of nitrogen application rate on soil microbial quantity and soil enzymes activities in maize/soybean intercropping systems
摘要
Abstract
To study the effects of nitrogen rate on soil microbial quantity and enzyme activity in maize/soybean intercropping system, a 2-factor design was developed. The main factor in the design was planting pattern, which included monoculturemaize (MM), sole soybean (SS) and maize/soybean relay strip intercropping system (IMS). Then the sub-factor of nitrogen application rate N for maize and soybean were no nitrogen application [0 kg(N)·hm-2, NN], reduced nitrogen application [180 kg(N)·hm-2, RN], and conventional nitrogen application [240 kg(N)·hm-2, CN]. The results showed that the quantities of fungi and actinomycetes in rhizosphere soil of intercropped maize were 25.37% and 8.79% higher than those of mono-culture maize, respectively. Also the quantities of soil fungi, actinomycetes and nitrogen-fixing bacteria in the rhizosphere soil of intercropped soybean were higher than those of sole soybean. Soil protease and urease activities in the rhizosphere soil of intercropped maize and protease activity in rhizosphere soil of intercropped soybean significantly increased compared with the corresponding monoculture. Under different nitrogen application rates, the quantities of soil fungi, actinomycetes and nitro-gen-fixing bacteria in the rhizosphere of maize and soybean were higher under RN than under NN and CN, respectively. In addition, nitrogen application increased the quantity of actinomycetes in the rhizosphere soil of maize and soybean. The quan-tities of nitrogen-fixing bacteria in the rhizosphere soil of maize were 17.78% and 5.67% higher under RN compared with those under NN and CN, respectively. Moreover, the activities of soil protease and urease in the rhizosphere of maize and ac-tivity of soil urease in the rhizosphere of soybean were highest under RN compared with those under NN and CN. Reduced nitrogen fertilization increased the degree of soil microbial and enzyme activities in the rhizosphere of maize/soybean relay strip intercropping system, promoted nitrogen uptake of maize and soybean, and thereby saved fertilizer use.关键词
玉米/大豆套作/施氮量/根际土壤/真菌/放线菌/固氮菌/土壤酶活性Key words
Maize/soybean relay strip intercropping/Nitrogen application rate/Rhizosphere soil/Fungi/Actinomycetes/Ni-trogen-fixing bacteria/Soil enzyme activity分类
农业科技引用本文复制引用
付智丹,周丽,陈平,杜青,庞婷,杨文钰,雍太文..施氮量对玉米/大豆套作系统土壤微生物数量及土壤酶活性的影响[J].中国生态农业学报,2017,25(10):1463-1474,12.基金项目
国家自然科学基金面上项目(31271669, 31671625)和国家重点研发计划(2016YFD0300202)资助 This study was supported by the National Natural Science Foundation of China (31271669, 31671625) and the National Key R & D Plan of China (2016YFD0300202). （31271669, 31671625）
