山西农业大学学报（自然科学版）2017，Vol.37Issue(10)：726-732,7.
马身猪ZNF280D基因新型剪接体的鉴定及生物信息学分析
Identification and bioinformatics analysis of alternative splicing isoforms of ZNF280D gene in Mashen Pig
摘要
Abstract
[Objective]In this study ,the splice variant of the gene of zinc finger protein 280D (ZNF280D) in Mashen pigs was investigated .[Methods]Based on the prediction of the ZNF280D gene splicing position in the pig transcrip-tome sequence ,these splice sites were verified by RT-PCR amplification ,cloning and sequencing technique .[Results] Two alternative splicing isoforms of the ZNF280D gene called ZNF280D1 and ZNF280D2 were discoveried in Mashen pigs .Compared with ZNF280D1 ,the exon 8 in ZNF280D2 was missed .Three C2 H2 zinc finger domain structures were found in ZNF280D1 that was a tC2 H2 type zinc finger protein .Four C2 H2 zinc finger domain structures were found in ZNF280D2 that was a maC2 H2 type zinc finger protein .[Conclusion]The greater affinity for DNA was detec-ted in ZN F280D2 .关键词
猪/ZNF280D基因/剪接体Key words
Sus scro f a/ZNF 280D gene/Alternative splicing isoforms分类
生物科学引用本文复制引用
靳玉舒,李萌,张旗,高鹏飞,李步高,郭晓红..马身猪ZNF280D基因新型剪接体的鉴定及生物信息学分析[J].山西农业大学学报（自然科学版）,2017,37(10):726-732,7.基金项目
山西省科技创新重点团队项目(201605D131045-24) （201605D131045-24）
山西省农业科技攻关项目(20140311020-5) （20140311020-5）
