山西农业大学学报（自然科学版）2017，Vol.37Issue(10)：733-742,10.
不同沙棘果渣添加水平对公绵羊睾丸和附睾抗氧化性的影响
Effects of supplementation of different sea buckthorn pomace levels on antioxidation in testis and epi-didymis of ram
摘要
Abstract
[Objective]The purpose of this study was to investigate the effects of dietary Sea buckthorn Pomace on an-tioxidation in testis and epididymis of ram .[Methods]A total of 24 Dopor × Small Tail Han Sheep ram lambs (5 month old ,25.00 ± 1.00 kg)were randomly selected and equally divided into 4 groups ,and received diet containing different level of sea buckthorn pomace (0% ,10% ,20% ,30% ) .At the end of the trial (65 d) ,the samples of testis and epi-didymis were collected and the mRNA and protein expression were detected .[Results]The results were as follows :The mRNA expression of GPx4 in testis was significantly lower in 30% group than that in control group and 10% supple-mentation group (P< 0.05) .Compared with the 10% and 20% groups ,the mRNA expression of Cu-ZnSOD in 30%group was significantly lower in caput epididymidis (P<0.05) .The mRNA expression of GPx4 in caput epididymidis was significantly lower in 30% supplementation group than this in control group (P<0.05) .Compared with the group with 10% sea buckthorn pomace ,the mRNA expression of CA T in 30% group was significantly lower in corpus epi-didymidis (P<0.05) .The mRNA expression of Cu-ZnSOD in cauda epididymidis was significantly lower in control group and 30% group than that in 20% sea buckthorn pomace supplementation group (P<0.05) .In addition ,compared with 10% sea buckthorn pomace addition group ,the mRNA expression of Cu-ZnSOD in 30% group was signifi-cantly lower in cauda epididymidis (P<0.05) .The protein expression of Nrf2 in caput epididymal and Cu-ZnSOD in the cauda epididymal were significantly higher in 20% group than that in 10% group and 30% group (P<0.05) .[Con-clusion]The results indicated that supplementation of 10% and 20% of dietary sea buckthorn pomace could improve the mRNA expression of antioxidation related genes expression in testis and epididymis of ram ,whereas supplementation of 30% sea buckthorn pomace reduced the mRNA expression of G Px4 in testis ,Cu-ZnSOD and G Px4 in caput epidid-ymidis ,CA T in corpus epididymidis and Cu-ZnSOD in cauda epididymidisa and the protein expression of Nrf2 in caput epididymidis and Cu-ZnSOD in cauda epididymidisa .Therefore ,dietary 30% sea buckthorn pomace is not optimal .关键词
沙棘果渣/睾丸/附睾/抗氧化/绵羊/mRNAKey words
Sea buckthorn pomace/Testis/Epididymis/Antioxidation/Ram/mRNA分类
农业科技引用本文复制引用
韩晓强,张春香,张文佳,项斌伟,张建新..不同沙棘果渣添加水平对公绵羊睾丸和附睾抗氧化性的影响[J].山西农业大学学报（自然科学版）,2017,37(10):733-742,10.基金项目
国家现代肉羊技术体系专项(CARS-39) （CARS-39）
