西北林学院学报2017，Vol.32Issue(5)：154-160,7.DOI:10.3969/j.issn.1001-7461.2017.05.27
基于PCR-DGGE技术的3种植食性叶蜂幼虫肠道细菌群落结构分析
Gut Bacteria Flora from Three Kinds of Herbivorous Sawfly Larvae Based on PCR-DGGE Technology
摘要
Abstract
Gut bacteria play an important role in coevolution between herbivorous insects and their host plants.In this paper the bacterial communities from larvae of three herbivorous sawfly species,Nematus hequensis,Pristiphora erichsonii and Acantholyda posticalis,were profiled by using 16S rRNA PCR-denaturing gradient gel electrophoresis (PCR-DGGE) technology,aiming to compare the characteristics of gut bacterial communities among sawflies feeding on different host trees.The bacterial communities of three sawfly larvae showed obvious difference,the gut bacterial community in N.hequensis which feed on the leaves of deciduous tree was clearly different from those in two sawflies (P.erichsonii and A.posticalis) feeding pine needles,both of which showed more similarity in gut bacterial community.Eight genera of Proteobacteria,Firmicutes and Actinobacteria were detected in N.hequensis,and Serratia and Rhizobium were the most abundant genera.Six genera of Proteobacteria and Firmicutes were identified in P.erichsonii that was dominated by Enterobacter and Pseudomonas.The bacterial flora of A.posticali was dominated by Wolbachia and Pseudomonas,while other five genera of Proteobacteria,Firmicutes and Tenericutes were detected in low abundance.So the bacterial compositions in three sawflies species are closely related with their feeding host trees,and our results would provide basic information to further research the roles of gut microbes in host selection for herbivorous insects.关键词
河曲丝叶蜂/落叶松叶蜂/松阿扁叶蜂/肠道细菌/PCR-DGGEKey words
Nematus hequensis/Pristiphora erichsonii/Acantholyda posticalis/gut bacteria/PCR-DGGE分类
农业科技引用本文复制引用
张帅帅,南小宁,王云果,朱兰芳,贺虹..基于PCR-DGGE技术的3种植食性叶蜂幼虫肠道细菌群落结构分析[J].西北林学院学报,2017,32(5):154-160,7.基金项目
中央高校基本科研业务费专项资金资助(QN2013081). （QN2013081）
